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Compton, 1990 - Google Patents

Degenerate primers for DNA amplification

Compton, 1990

Document ID
4770113025344566982
Author
Compton T
Publication year
Publication venue
PCR protocols: a guide to methods and applications

External Links

Snippet

A mixture of oligonucleotides varying in base sequence but with the same number of bases (" degenerate") can be substituted for defined sequence oligonucleotides as primers and successfully coupled with PCR, resulting in the desired gene-specific amplification products …
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
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