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/*==========================================================================
SeqAn - The Library for Sequence Analysis
http://www.seqan.de
============================================================================
Copyright (C) 2007
This library is free software; you can redistribute it and/or
modify it under the terms of the GNU Lesser General Public
License as published by the Free Software Foundation; either
version 3 of the License, or (at your option) any later version.
This library is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the GNU
Lesser General Public License for more details.
============================================================================
$Id$
==========================================================================*/
//SEQAN_NO_GENERATED_FORWARDS: no forwards are generated for this file
#ifndef SEQAN_HEADER_FILE_FORMAT_MMAP_H
#define SEQAN_HEADER_FILE_FORMAT_MMAP_H
namespace SEQAN_NAMESPACE_MAIN
{
// define memory mapped stringset
typedef StringSet<String<char, MMap<> >, Owner<ConcatDirect<> > > MultiFasta; //deprecated
typedef StringSet<String<char, MMap<> >, Owner<ConcatDirect<> > > MultiSeqFile;
template <typename TValue>
inline bool
_isLineBreak(TValue value)
{
return (value == '\n' || value == '\r');
}
template <typename TIterator>
inline bool
_seekLineBreak(TIterator &it, TIterator itEnd)
{
while (!_isLineBreak(*it))
if (++it == itEnd) return false;
return true;
}
template <typename TIterator>
inline bool
_seekNonLineBreak(TIterator &it, TIterator itEnd)
{
if (*it == '\n')
{
if (++it == itEnd) return false;
if (*it == '\r')
if (++it == itEnd) return false;
} else
if (*it == '\r')
{
if (++it == itEnd) return false;
if (*it == '\n')
if (++it == itEnd) return false;
}
return true;
}
template <typename TIterator>
inline bool
_seekTab(TIterator& it, TIterator itEnd)
{
for (; it != itEnd; ++it)
if (*it == '\t') return true;
return false;
}
//////////////////////////////////////////////////////////////////////////////
// File Formats - Fasta
//////////////////////////////////////////////////////////////////////////////
// test for Fasta format
template < typename TSeq >
inline bool
guessFormat(
TSeq const & seq,
Fasta)
{
return seq[0] == '>';
}
template < typename TFilename >
inline bool
guessFormatFromFilename(
TFilename const & fname,
Fasta)
{
typedef typename Value<TFilename>::Type TValue;
typedef ModifiedString<TFilename, ModView<FunctorLowcase<TValue> > > TLowcase;
typename Size<TFilename>::Type len = length(fname);
TLowcase lowcaseFname(fname);
if (len >= 3)
{
if (suffix(lowcaseFname, len - 3) == ".fa") return true;
}
if (len >= 4)
{
if (suffix(lowcaseFname, len - 4) == ".fas") return true;
if (suffix(lowcaseFname, len - 4) == ".faa") return true; // FASTA Amino Acid file
if (suffix(lowcaseFname, len - 4) == ".ffn") return true; // FASTA nucleotide coding regions file
if (suffix(lowcaseFname, len - 4) == ".fna") return true; // FASTA Nucleic Acid file
if (suffix(lowcaseFname, len - 4) == ".frn") return true;
}
if (len >= 6)
{
if (suffix(lowcaseFname, len - 6) == ".fasta") return true;
}
return false;
}
// split stringset into single Fasta sequences
template < typename TValue, typename TConfig, typename TDelimiter >
inline void
split(
StringSet<String<TValue, MMap<TConfig> >, Owner<ConcatDirect<TDelimiter> > > &me,
Fasta)
{
typedef String<TValue, MMap<TConfig> > TString;
typedef StringSet<TString, ConcatDirect<TDelimiter> > TStringSet;
typedef typename Iterator<TString const, Standard>::Type TIterator;
clear(me.limits);
TIterator itBeg = begin(me.concat, Standard());
TIterator itEnd = end(me.concat, Standard());
bool newLine = true;
for (TIterator it = itBeg; it != itEnd; ++it)
{
TValue c = *it;
if (newLine && c == '>')
appendValue(me.limits, it - itBeg, Generous());
newLine = _isLineBreak(c);
}
if (empty(me.limits))
appendValue(me.limits, 0);
appendValue(me.limits, itEnd - itBeg);
}
template <typename TSeq, typename TFastaSeq>
inline void
assignSeq(
TSeq & dst,
TFastaSeq const & fasta,
Fasta)
{
typedef typename Iterator<TFastaSeq const, Standard>::Type TIterator;
typedef typename Iterator<TSeq, Standard>::Type TDstIterator;
TIterator it = begin(fasta, Standard());
TIterator itEnd = end(fasta, Standard());
clear(dst);
// skip Fasta id
if (it == itEnd) return;
if (*it == '>')
{
if (!_seekLineBreak(it, itEnd)) return;
if (!_seekNonLineBreak(it, itEnd)) return;
}
// copy sequence
resize(dst, itEnd - it);
TDstIterator dit = begin(dst, Standard());
for (; it != itEnd; ++it)
if (!_isLineBreak(*it))
{
*dit = *it;
++dit;
}
resize(dst, dit - begin(dst, Standard()));
}
template <typename TSeq, typename TFastaSeq>
inline void
assignSeqId(
TSeq & dst,
TFastaSeq const & fasta,
Fasta)
{
typedef typename Iterator<TFastaSeq const, Standard>::Type TIterator;
TIterator itBeg = begin(fasta, Standard());
TIterator itEnd = end(fasta, Standard());
TIterator it = itBeg;
clear(dst);
if (it == itEnd) return;
if (*it == '>')
{
_seekLineBreak(it, itEnd);
assign(dst, infix(fasta, 1, it - itBeg));
}
}
template <typename TSeq, typename TFastaSeq>
inline void
assignQual(
TSeq & dst,
TFastaSeq const &,
Fasta)
{
clear(dst);
}
template <typename TSeq, typename TFastaSeq>
inline void
assignQualId(
TSeq & dst,
TFastaSeq const &,
Fasta)
{
clear(dst);
}
//////////////////////////////////////////////////////////////////////////////
// File Formats - Fastq (Fasta extension for quality values)
//////////////////////////////////////////////////////////////////////////////
/**
.Tag.File Format.tag.Fastq:
FASTQ file format for sequences.
*/
struct TagFastq_;
typedef Tag<TagFastq_> const Fastq;
// test for Fastq format
template < typename TSeq >
inline bool
guessFormat(
TSeq const & seq,
Fastq)
{
return seq[0] == '@';
}
template < typename TFilename >
inline bool
guessFormatFromFilename(
TFilename const & fname,
Fastq)
{
typedef typename Value<TFilename>::Type TValue;
typedef ModifiedString<TFilename, ModView<FunctorLowcase<TValue> > > TLowcase;
typename Size<TFilename>::Type len = length(fname);
TLowcase lowcaseFname(fname);
if (len >= 3)
{
if (suffix(lowcaseFname, len - 3) == ".fq") return true;
}
if (len >= 6)
{
if (suffix(lowcaseFname, len - 6) == ".fastq") return true;
}
return false;
}
// split stringset into single Fasta sequences
template < typename TValue, typename TConfig, typename TDelimiter >
inline void
split(
StringSet<String<TValue, MMap<TConfig> >, Owner<ConcatDirect<TDelimiter> > > &me,
Fastq)
{
typedef String<TValue, MMap<TConfig> > TString;
typedef StringSet<TString, ConcatDirect<TDelimiter> > TStringSet;
typedef typename Iterator<TString const, Standard>::Type TIterator;
clear(me.limits);
TIterator itBeg = begin(me.concat, Standard());
TIterator itEnd = end(me.concat, Standard());
bool newLine = true;
for (TIterator it = itBeg; it != itEnd; ++it)
{
if (newLine && *it == '@')
appendValue(me.limits, it - itBeg, Generous());
if (newLine && *it == '+')
{
// skip qualitity fasta id
if (!_seekLineBreak(it, itEnd)) break;
if (!_seekNonLineBreak(it, itEnd)) break;
// skip qualitity values
if (!_seekLineBreak(it, itEnd)) break;
}
newLine = _isLineBreak(*it);
}
if (empty(me.limits))
appendValue(me.limits, 0);
appendValue(me.limits, itEnd - itBeg);
}
template <typename TSeq, typename TFastaSeq>
inline void
assignSeq(
TSeq & dst,
TFastaSeq const & fasta,
Fastq)
{
typedef typename Iterator<TFastaSeq const, Standard>::Type TIterator;
typedef typename Iterator<TSeq, Standard>::Type TDstIterator;
TIterator it = begin(fasta, Standard());
TIterator itEnd = end(fasta, Standard());
clear(dst);
// skip Fasta id
if (it == itEnd) return;
if (*it == '@')
{
if (!_seekLineBreak(it, itEnd)) return;
if (!_seekNonLineBreak(it, itEnd)) return;
}
// copy sequence
resize(dst, itEnd - it);
TDstIterator dit = begin(dst, Standard());
for (; it != itEnd; ++it)
{
if (_isLineBreak(*it))
{
if (!_seekNonLineBreak(it, itEnd)) break;
if (*it == '+') break;
}
*dit = *it;
++dit;
}
resize(dst, dit - begin(dst, Standard()));
}
template <typename TSeq, typename TFastaSeq>
inline void
assignSeqId(
TSeq & dst,
TFastaSeq const & fasta,
Fastq)
{
typedef typename Iterator<TFastaSeq const, Standard>::Type TIterator;
TIterator itBeg = begin(fasta, Standard());
TIterator itEnd = end(fasta, Standard());
TIterator it = itBeg;
clear(dst);
if (it == itEnd) return;
if (*it == '@')
{
_seekLineBreak(it, itEnd);
assign(dst, infix(fasta, 1, it - itBeg));
}
}
template <typename TSeq, typename TFastaSeq>
inline void
assignQual(
TSeq & dst,
TFastaSeq const & fasta,
Fastq)
{
typedef typename Iterator<TFastaSeq const, Standard>::Type TIterator;
typedef typename Iterator<TSeq, Standard>::Type TDstIterator;
TIterator it = begin(fasta, Standard());
TIterator itEnd = end(fasta, Standard());
clear(dst);
if (it == itEnd) return;
if (*it == '@')
{
// seek quality id
do {
if (!_seekLineBreak(it, itEnd)) return;
if (!_seekNonLineBreak(it, itEnd)) return;
} while (*it != '+');
// skip quality id
if (!_seekLineBreak(it, itEnd)) return;
if (!_seekNonLineBreak(it, itEnd)) return;
// copy sequence
resize(dst, itEnd - it);
TDstIterator dit = begin(dst, Standard());
for (; it != itEnd; ++it)
if (!_isLineBreak(*it))
{
*dit = *it;
++dit;
}
resize(dst, dit - begin(dst, Standard()));
}
}
template <typename TSeq, typename TFastaSeq>
inline void
assignQualId(
TSeq & dst,
TFastaSeq const & fasta,
Fastq)
{
typedef typename Iterator<TFastaSeq const, Standard>::Type TIterator;
TIterator itBeg = begin(fasta, Standard());
TIterator itEnd = end(fasta, Standard());
TIterator it1 = itBeg;
clear(dst);
if (it1 == itEnd) return;
if (*it1 == '@')
{
do {
if (!_seekLineBreak(it1, itEnd)) return;
if (!_seekNonLineBreak(it1, itEnd)) return;
} while (*it1 != '+');
TIterator it2 = it1;
_seekLineBreak(it2, itEnd);
assign(dst, infix(fasta, (it1 - itBeg) + 1, it2 - itBeg));
}
}
//////////////////////////////////////////////////////////////////////////////
// File Formats - QSeq (used by Illumina for most of their read files)
//////////////////////////////////////////////////////////////////////////////
struct _QSeq;
typedef Tag<_QSeq> const QSeq;
// FIXME The following enum is more or less arbitrary since the information
// in a QSeq file may differ depending on where they come from. Not sure if
// this is something that needs to be fixed here, rather than in the Illu-
// mina pipeline itself.
//
// Also, this enum is quite convoluted but I don't feel like spilling a lot
// of common symbols (e.g. 'X', 'Y') into the SeqAn namespace.
struct QSeqEntry {
enum {
MachineName,
Run,
Lane,
Tile,
X,
Y,
Index,
Read,
Sequence,
Quality,
Filter
};
};
template < typename TString >
inline bool _isQSeqFile(TString const& filename)
{
unsigned int const namelen = 19;
unsigned int const pathlen = length(filename);
if (pathlen < namelen) return false;
::std::string str;
assign(str, suffix(filename, pathlen - namelen));
::std::istringstream is(str);
unsigned int num;
// Format (as regex): /^s_\d_\d_\d{4}_qseq.txt$/
return is.get() == 's' && is.get() == '_' &&
isdigit(is.get()) && is.get() == '_' &&
isdigit(is.get()) && is.get() == '_' &&
is >> num &&
getline(is, str) && str == "_qseq.txt" && is.eof();
}
// // Needed?
// template < typename TString >
// inline TString _getFirstFile(
// char const* dirname,
// QSeq)
// {
// Directory dir(dirname);
//
// for ( ; dir; ++dir)
// if (_isQSeqFile(*dir))
// return *dir;
// }
//
// template < typename TString >
// inline String< TString >
// _getAllFiles(
// char const* dirname,
// QSeq)
// {
// String< TString > ret;
// Directory dir(dirname);
//
// for (; dir; ++dir)
// if (_isQSeqFile(*dir))
// appendValue(ret, *dir);
//
// return ret;
// }
// test for QSeq format
template < typename TSeq >
inline bool
guessFormat(
TSeq const & seq,
QSeq)
{
typedef typename Iterator<TSeq const>::Type TIter;
TIter front = begin(seq);
TIter const back = end(seq);
if (!_seekTab(front, back)) return false;
::std::string token_base;
assign(token_base, seq);
::std::istringstream is(token_base);
::std::string mname;
unsigned int numval;
// Actual information encoded in qseq file may vary. Take a few guesses:
// machine name, run number, lane number, tile number
return is >> mname >> numval >> numval >> numval;
}
template < typename TFilename >
inline bool
guessFormatFromFilename(
TFilename const & fname,
QSeq)
{
// QSeq files come in a variety of ways throughout the Gerald pipeline.
// In this simplest case, "sorted.txt" is a file in a fragment genome
// directory, each corresponding to 10MB worth of DNA.
static CharString const standalone_name = "sorted.txt";
typename Size<TFilename>::Type len = length(fname);
return len >= length(standalone_name) &&
suffix(fname, len - length(standalone_name)) == standalone_name ||
_isQSeqFile(fname);
}
// split stringset into single QSeq sequences
template < typename TValue, typename TConfig, typename TDelimiter >
inline void
split(
StringSet<String<TValue, MMap<TConfig> >, Owner<ConcatDirect<TDelimiter> > > &me,
QSeq)
{
typedef String<TValue, MMap<TConfig> > TString;
typedef StringSet<TString, ConcatDirect<TDelimiter> > TStringSet;
typedef typename Iterator<TString const, Standard>::Type TIterator;
clear(me.limits);
TIterator const front = begin(me.concat, Standard());
TIterator const back = end(me.concat, Standard());
appendValue(me.limits, 0, Generous());
for (TIterator i = front; i != back; ++i)
if (_isLineBreak(*i))
appendValue(me.limits, i - front, Generous());
if (!_isLineBreak(*(back - 1))) // Ignore final line break.
appendValue(me.limits, back - front);
}
template <typename TSequence, typename TSource>
void assignQSeqEntry(
TSequence& destination,
TSource const& source,
unsigned int entry
) {
typedef typename Iterator<TSource const>::Type TIterator;
TIterator const front = begin(source, Standard());
TIterator const back = end(source, Standard());
TIterator infixStart = front;
for (unsigned int i = QSeqEntry::MachineName; i < entry; ++i) {
_seekTab(infixStart, back);
++infixStart;
}
SEQAN_ASSERT(infixStart != back);
TIterator infixEnd = infixStart + 1;
_seekTab(infixEnd, back);
assign(destination, infix(source, infixStart - front, infixEnd - front));
}
template <typename TSeq, typename TQSeqSeq>
inline void
assignSeq(
TSeq & dst,
TQSeqSeq const & fasta,
QSeq)
{
assignQSeqEntry(dst, fasta, QSeqEntry::Sequence);
}
template <typename TSeq, typename TQSeqSeq>
inline void
assignSeqId(
TSeq & dst,
TQSeqSeq const & fasta,
QSeq)
{
// For now: just return the whole line.
typename Position<TQSeqSeq const>::Type front = 0;
while (_isLineBreak(fasta[front]))
++front;
assign(dst, infix(fasta, front, length(fasta)));
}
template <typename TSeq, typename TQSeqSeq>
inline void
assignQual(
TSeq & dst,
TQSeqSeq const & fasta,
QSeq)
{
assignQSeqEntry(dst, fasta, QSeqEntry::Quality);
}
template <typename TSeq, typename TQSeqSeq>
inline void
assignQualId(
TSeq & dst,
TQSeqSeq const & fasta,
QSeq)
{
assignSeqId(dst, fasta, QSeq());
}
//////////////////////////////////////////////////////////////////////////////
// File Formats - Auto-Format
//////////////////////////////////////////////////////////////////////////////
typedef
TagList<Fastq,
TagList<Fasta,
TagList<QSeq> > > SeqFormats;
typedef TagSelector<SeqFormats> AutoSeqFormat;
//____________________________________________________________________________
// guess file format
template < typename TFileSeq >
inline bool
guessFormat(
TFileSeq const &,
TagSelector<> &format)
{
format.tagId = 0;
return false;
}
template < typename TFileSeq, typename TTagList >
inline bool
guessFormat(
TFileSeq const & seq,
TagSelector<TTagList> &format)
{
if (guessFormat(seq, typename TTagList::Type()))
{
if (format.tagId == 0)
{
format.tagId = Length<TTagList>::VALUE; // if tagId == 0 then store detected format
return true;
} else
return format.tagId == Length<TTagList>::VALUE; // if tagId != 0 then compare detected format with tagId
}
return guessFormat(seq, static_cast<typename TagSelector<TTagList>::Base &>(format));
}
//____________________________________________________________________________
// guess file format from filename
template < typename TFilename >
inline bool
guessFormatFromFilename(
TFilename const &,
TagSelector<> &format)
{
format.tagId = 0;
return false;
}
template < typename TFilename, typename TTagList >
inline bool
guessFormatFromFilename(
TFilename const & fname,
TagSelector<TTagList> &format)
{
if (guessFormatFromFilename(fname, typename TTagList::Type()))
{
if (format.tagId == 0)
{
format.tagId = Length<TTagList>::VALUE; // if tagId == 0 then store detected format
return true;
} else
return format.tagId == Length<TTagList>::VALUE; // if tagId != 0 then compare detected format with tagId
return true;
}
return guessFormatFromFilename(fname, static_cast<typename TagSelector<TTagList>::Base &>(format));
}
//____________________________________________________________________________
// split stringset into single sequences
template < typename TValue, typename TConfig, typename TDelimiter >
inline void
split(
StringSet<String<TValue, MMap<TConfig> >, Owner<ConcatDirect<TDelimiter> > > &,
TagSelector<void> const &)
{
}
template < typename TValue, typename TConfig, typename TDelimiter, typename TTagList >
inline void
split(
StringSet<String<TValue, MMap<TConfig> >, Owner<ConcatDirect<TDelimiter> > > &me,
TagSelector<TTagList> const &format)
{
if (format.tagId == Length<TTagList>::VALUE)
split(me, typename TTagList::Type());
else
split(me, static_cast<typename TagSelector<TTagList>::Base const &>(format));
}
//____________________________________________________________________________
// assignSeq
template <typename TSeq, typename TFileSeq>
inline void
assignSeq(
TSeq &,
TFileSeq const &,
TagSelector<> const &)
{
}
template <typename TSeq, typename TFileSeq, typename TTagList>
inline void
assignSeq(
TSeq & dst,
TFileSeq const & seq,
TagSelector<TTagList> const &format)
{
if (format.tagId == Length<TTagList>::VALUE)
assignSeq(dst, seq, typename TTagList::Type());
else
assignSeq(dst, seq, static_cast<typename TagSelector<TTagList>::Base const &>(format));
}
//____________________________________________________________________________
// assignSeqId
template <typename TSeqId, typename TFileSeq>
inline void
assignSeqId(
TSeqId &,
TFileSeq const &,
TagSelector<> const &)
{
}
template <typename TSeqId, typename TFileSeq, typename TTagList>
inline void
assignSeqId(
TSeqId & dst,
TFileSeq const & seq,
TagSelector<TTagList> const &format)
{
if (format.tagId == Length<TTagList>::VALUE)
assignSeqId(dst, seq, typename TTagList::Type());
else
assignSeqId(dst, seq, static_cast<typename TagSelector<TTagList>::Base const &>(format));
}
//____________________________________________________________________________
// assignQual
template <typename TSeq, typename TFileSeq>
inline void
assignQual(
TSeq &,
TFileSeq const &,
TagSelector<> const &)
{
}
template <typename TSeq, typename TFileSeq, typename TTagList>
inline void
assignQual(
TSeq & dst,
TFileSeq const & seq,
TagSelector<TTagList> const &format)
{
if (format.tagId == Length<TTagList>::VALUE)
assignQual(dst, seq, typename TTagList::Type());
else
assignQual(dst, seq, static_cast<typename TagSelector<TTagList>::Base const &>(format));
}
//____________________________________________________________________________
// assignQualId
template <typename TSeq, typename TFileSeq>
inline void
assignQualId(
TSeq &,
TFileSeq const &,
TagSelector<> const &)
{
}
template <typename TSeq, typename TFileSeq, typename TTagList>
inline void
assignQualId(
TSeq & dst,
TFileSeq const & seq,
TagSelector<TTagList> const &format)
{
if (format.tagId == Length<TTagList>::VALUE)
assignQualId(dst, seq, typename TTagList::Type());
else
assignQualId(dst, seq, static_cast<typename TagSelector<TTagList>::Base const &>(format));
}
//////////////////////////////////////////////////////////////////////////////
// Directory import
//////////////////////////////////////////////////////////////////////////////
template <typename TSeqSet, typename TFilename, typename TSeqFormat>
inline void
appendSeqs(
TSeqSet &seqSet,
TFilename &dirname,
TSeqFormat format)
{
typedef typename Value<TSeqSet>::Type TSeq;
Directory dir(dirname);
TSeq seq;
MultiSeqFile multiSeqFile;
if (!atEnd(dir))
{
// dirname is path of a directory
CharString fname = dirname;
#ifdef PLATFORM_WINDOWS
appendValue(fname, '\\');
#else
appendValue(fname, '/');
#endif
size_t len = length(fname);
for (; !atEnd(dir); goNext(dir))
{
assign(suffix(fname, len), value(dir));
if (guessFormatFromFilename(fname, format))
{
std::cout << fname<<std::endl;
if (!open(multiSeqFile.concat, toCString(fname), OPEN_RDONLY)) continue;
split(multiSeqFile, format);
unsigned seqCount = length(multiSeqFile);
reserve(seqSet, length(seqSet) + seqCount, Generous());
for(unsigned i = 0; i < seqCount; ++i)
{
assignSeq(seq, multiSeqFile[i], format);
appendValue(seqSet, seq, Generous());
}
close(multiSeqFile.concat);
}
}
}
else
{
// dirname is path of a file
if (guessFormatFromFilename(dirname, format))
{
if (!open(multiSeqFile.concat, toCString(dirname), OPEN_RDONLY)) return;
split(multiSeqFile, format);
unsigned seqCount = length(multiSeqFile);
reserve(seqSet, length(seqSet) + seqCount, Generous());
for(unsigned i = 0; i < seqCount; ++i)
{
assignSeq(seq, multiSeqFile[i], format);
appendValue(seqSet, seq, Generous());
}
close(multiSeqFile.concat);
}
}
}
}
#endif
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