MANTI is a one-stop shop N-termini annotation & evaluation solution. MANTI was previously (un)known as muda.pl ahead of v3.7, the project was renamed to MANTI.pl with v3.7 on 2019-06-24.
It congregates information from different MaxQuant or DiaNN/MSFragger output files into a master file suitable explicitly for protein neo-termini analyses. The central anchor for the data congregation is the modificationSpecificPeptides.txt or diann-output.pr_matrix.tsv file - additional data is inferred from different other source files from the corresponding folder. Maybe also useful for normal proteomics purposes but this script is heavily optimized for protein neo-termini identification and validation. A graphical interface is available as Yoğurtlu_MANTI (a Perl/Tk script) + execut. application versions for Win1x without the need to have Perl installed locally.
For a very detailed explanation of script parameters and the evaluation strategy, please consult the extensive manual PDF
Features
- Mastering Advanced N-Termini Interpretation
- MaxQuant N-termini evaluation
- DiaNN/MSFragger DIA N-termini data validation & analysis
- Protein-Termini evaluation & validation
- (UniProt) data annotation & assembly
- Limma, LOCALIZER, TargetP2.0 & TopFINDer integration
- Re-mapping of identified Termini to correct Isoforms
- Plenty of visualization options with supplied R scripts
Project Samples
