Crossbow's minor adaptation of the SoapSNP output format has lots of useful info, but it's apparently missing a piece key to further annotation: strand. Tools like EBI's SNP Effect Predictor http://uswest.ensembl.org/info/website/upload/index.html#Consequence want it -- obviously, the effect on protein coding is different; upstream/downstream distance criteria, etc. You have this information internally, because Bowtie produces it. So how about adding another column?
Cheers!
Howie Goodell
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Hi --
Please cancel this request; my misunderstanding. I realize strand info for follow-on packages should always be "+"; since you convert position and complement bases where you align reads from the reverse strand.
Thanks!
Howie Goodell