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WO2018148853A1 - Non-invasive device for taking genetic samples - Google Patents

Non-invasive device for taking genetic samples Download PDF

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Publication number
WO2018148853A1
WO2018148853A1 PCT/CL2017/000004 CL2017000004W WO2018148853A1 WO 2018148853 A1 WO2018148853 A1 WO 2018148853A1 CL 2017000004 W CL2017000004 W CL 2017000004W WO 2018148853 A1 WO2018148853 A1 WO 2018148853A1
Authority
WO
WIPO (PCT)
Prior art keywords
thickness
substrates
substrate
layer
epithelial cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/CL2017/000004
Other languages
Spanish (es)
French (fr)
Inventor
Gabriel Edgardo ORTEGA PERALTA
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to PCT/CL2017/000004 priority Critical patent/WO2018148853A1/en
Publication of WO2018148853A1 publication Critical patent/WO2018148853A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
    • A61B5/00—Measuring for diagnostic purposes; Identification of persons
    • A61B5/117—Identification of persons
    • A61B5/1171—Identification of persons based on the shapes or appearances of their bodies or parts thereof
    • A61B5/1174—Identification of persons based on the shapes or appearances of their bodies or parts thereof using footprinting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C65/00—Joining or sealing of preformed parts, e.g. welding of plastics materials; Apparatus therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B29—WORKING OF PLASTICS; WORKING OF SUBSTANCES IN A PLASTIC STATE IN GENERAL
    • B29C—SHAPING OR JOINING OF PLASTICS; SHAPING OF MATERIAL IN A PLASTIC STATE, NOT OTHERWISE PROVIDED FOR; AFTER-TREATMENT OF THE SHAPED PRODUCTS, e.g. REPAIRING
    • B29C67/00—Shaping techniques not covered by groups B29C39/00 - B29C65/00, B29C70/00 or B29C73/00
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B32—LAYERED PRODUCTS
    • B32B—LAYERED PRODUCTS, i.e. PRODUCTS BUILT-UP OF STRATA OF FLAT OR NON-FLAT, e.g. CELLULAR OR HONEYCOMB, FORM
    • B32B7/00—Layered products characterised by the relation between layers; Layered products characterised by the relative orientation of features between layers, or by the relative values of a measurable parameter between layers, i.e. products comprising layers having different physical, chemical or physicochemical properties; Layered products characterised by the interconnection of layers

Definitions

  • the present application is directed to a procedure for the determination of molecular identities and pathologies from the AON of epithelial cells retained in a device. Specifically, this device fulfills the function of capturing said cells and keeping them stable and unalterable over time.
  • DNA is obtained with the method of drawing blood or saliva by means of buccal isopo, considered intrusive to the human body. These methods are causing serious inconveniences both from a technical and legal point of view.
  • the device consists of an adhesive sheet consisting of substrates on the lower and upper face, with adhesive acrylate in the middle, which involves taking the fingerprint, palmar or plantal with a very simple and safe method in a non-intrusive way, allowing its preservation Over time, it remains unchanged without the need for any external method at room temperature.
  • Figure 1 Represents a side view of the structure of the device of the present application. Detailed description of the invention
  • the water-based adhesives are an acrylate (2) and the substrates (1) and (3) are polymeric sheets.
  • the adhesive apa has a thickness no greater than 40 microns.
  • the thickness of the substrates (1) and (3) is greater than the thickness of the adhesive or acrylate layer and (the substrates have a thickness no greater than 60 microns.
  • the device is composed of a white support substrate (1), on which an aqueous base adhesive layer (2) is arranged and then a transparent cover substrate (3) is provided.
  • Said structure of this device allows the capture of the fingerprint, palmar or plantal of any individual, leaving in said posture its genetic or genetic characteristics by the segregation of epithelial cells, obtaining as previously expressed DNA of that person in good quality and quantity to then take the laboratory simply and safely to perform the corresponding studies; either for your identity or genetic pathologies through the sampling device; and extract the genetic material from said device. Please, using a reagent KIT.
  • the devices are composed as follows: White polymer layer (1), not more than 60 microns thick, water-based acrylate adhesive layer (2) not greater than 40 microns and layer (3) transparent polymeric sheet not exceeding 60 microns thick.

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Medical Informatics (AREA)
  • Molecular Biology (AREA)
  • Surgery (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to a device for capturing, collecting and preserving human DNA from epithelial cells of fingerprints, palm prints, or footprints. The invention also relates to a non-invasive method for providing filial identities and genetic disorders using a device with an adhesive sheet that does not change over time and is easy to preserve.

Description

Dispositivo de toma de muestras genéticas no intrusivo  Non-intrusive genetic sampling device

La presente solicitud está dirigida a un procedimiento para la determinación de identidades y patologías moleculares a partir del AON de células epiteliales retenidas en un dispositivo. Específicamente, este dispositivo cumple la función de capturar dichas células y mantenerlas estables e inalterables en el tiempo.  The present application is directed to a procedure for the determination of molecular identities and pathologies from the AON of epithelial cells retained in a device. Specifically, this device fulfills the function of capturing said cells and keeping them stable and unalterable over time.

Estado de la técnica  State of the art

En la actualidad, el ADN se obtiene con el método tanto de sacar sangre o saliva por medio de isopo bucal, considerados estos intrusivos al cuerpo humano. Dichos métodos son causantes de serios inconvenientes tanto desde el punto de vista técnico, como legal.  At present, DNA is obtained with the method of drawing blood or saliva by means of buccal isopo, considered intrusive to the human body. These methods are causing serious inconveniences both from a technical and legal point of view.

En el técnico, existen inconvenientes graves como:  In the technician, there are serious problems such as:

• Contagio de enfermedades.  • Disease transmission.

• Traslado en cadena de conservación.  • Transfer in conservation chain.

• Distorsión o cambio de muestras.  • Distortion or change of samples.

En el campo legal:  In the legal field:

• Custodia en el traslado.  • Custody in the transfer.

• Dificultad en la toma.  • Difficulty taking.

• Utilización de personal capacitado.  • Use of trained personnel.

El dispositivo consiste en una lámina adhesiva constituida por sustratos en la cara inferior y superior, con acrilato adhesivado en el medio, que implica tomar la huella genética dactilar, palmar o plantal con un método muy simple y seguro de forma no intrusiva, permitiendo su conservación a través del tiempo, que permanece inalterable sin necesidad de ningún método externo a temperatura ambiente.  The device consists of an adhesive sheet consisting of substrates on the lower and upper face, with adhesive acrylate in the middle, which involves taking the fingerprint, palmar or plantal with a very simple and safe method in a non-intrusive way, allowing its preservation Over time, it remains unchanged without the need for any external method at room temperature.

Utilidades Utilities

1- Identificación genética masiva a muy bajo costo.  1- Massive genetic identification at a very low cost.

2- Actas de nacimiento.  2- Birth certificates.

3- Actas de defunción.  3- Death certificates.

4- Actas de matrimonio.  4- Marriage records.

5- Identificación de reclusos y visitantes en Penitenciaría.  5- Identification of inmates and visitors in Penitentiary.

6- Alerta a Policías de criminales buscados.  6- Alert Police of wanted criminals.

7- Centro de identificación científica.  7- Center for scientific identification.

8- Prevención de enfermedades heredadas genéticamente.  8- Prevention of genetically inherited diseases.

9- Veracidad y precisión genética en los diagnósticos.  9- Genetic accuracy and precision in diagnoses.

10- Guarda genética familiar  10- Save family genetics

11- Extravío y cambios de bebés.  11- Loss and changes of babies.

Breve descripción de las figuras. Brief description of the figures.

Figura 1: Representa una vista lateral de la estructura del dispositivo de la presente solicitud. Descripción detallada de la invención Figure 1: Represents a side view of the structure of the device of the present application. Detailed description of the invention

El dispositivo para la captura, conservación y extracción de células epiteliales del cual obtenemos ADN de buena calidad para realizar estudios posteriores con utilidad identificatoria y diagnostica la calidad del ADN obtenido y purificado. Permite su amplificación por el método de PCR. The device for capturing, preserving and extracting epithelial cells from which we obtain good quality DNA for further studies with identifying utility and diagnoses the quality of the DNA obtained and purified. It allows its amplification by the PCR method.

Los adhesivos de base acuosa son un acrilato (2) y os sustratos (1) y (3) son láminas poliméricas. La apa de adhesivo tiene un espesor no mayor a 40 micrones. El espesor de los sustratos (1) y (3) es mayor que el espesor de la capa de adhesivo o acrilato y (os sustratos tienen un espesor no mayor a 60 micrones.  The water-based adhesives are an acrylate (2) and the substrates (1) and (3) are polymeric sheets. The adhesive apa has a thickness no greater than 40 microns. The thickness of the substrates (1) and (3) is greater than the thickness of the adhesive or acrylate layer and (the substrates have a thickness no greater than 60 microns.

El dispositivo se compone por un sustrato (1) de soporte color blanco, sobre el cual se dispone una capa dé adhesivo de base acuosa (2) y luego se dispone un sustrato cobertor (3) transparente.  The device is composed of a white support substrate (1), on which an aqueous base adhesive layer (2) is arranged and then a transparent cover substrate (3) is provided.

Dicha estructura de este dispositivo permite la captura de la huella digital, palmar o plantal de cualquier individuo, dejando en dicha postura sus características génicas o genéticas por la segregación de células epiteliales, obteniendo como expresamos anteriormente ADN de esa persona en buena calidad y cantidad para luego llevar a laboratorio en forma simple y segura para realizar los estudios correspondientes; ya sea para su identidad o patologías genéticas por medio del dispositivo de toma de muestra; y extraer el material genético de dicho dispositivo. Por ¡sopado, utilizando un KIT de reactivos. Said structure of this device allows the capture of the fingerprint, palmar or plantal of any individual, leaving in said posture its genetic or genetic characteristics by the segregation of epithelial cells, obtaining as previously expressed DNA of that person in good quality and quantity to then take the laboratory simply and safely to perform the corresponding studies; either for your identity or genetic pathologies through the sampling device; and extract the genetic material from said device. Please, using a reagent KIT.

Ejemplo: Example:

De acuerdo a los estudios realizados en laboratorio, los dispositivos se componen de la siguiente manera: Capa (1) lámina polimérica de color blanco, no mayor a un espesor de 60 micrones, capa (2) adhesivo acrilato de base acuosa no mayor a 40 micrones y capa (3) lámina polimérica transparente de espesor no mayor a 60 micrones.  According to laboratory studies, the devices are composed as follows: White polymer layer (1), not more than 60 microns thick, water-based acrylate adhesive layer (2) not greater than 40 microns and layer (3) transparent polymeric sheet not exceeding 60 microns thick.

Claims

Reivindicaciones Claims 1- Dispositivo de toma de muestras de ADN no intrusiva al cuerpo humano, extraído por células epiteliales que permiten conservar dichas muestras por largo tiempo. 1- Device for sampling non-intrusive DNA to the human body, extracted by epithelial cells that allow these samples to be preserved for a long time. CARACTERIZADO porque comprende: un sustrato de soporte (1) sobre el cual se dispone una capa de adhesivo de base acuosa (2) y sobre dicha capa (2) se dispone un sustrato cobertor (3)  CHARACTERIZED because it comprises: a support substrate (1) on which an aqueous-based adhesive layer (2) is arranged and on said layer (2) a cover substrate (3) is provided 2- Dispositivo de acuerdo a la reivindicación 1, CARACTERIZADO porque los el adhesivo de base acuosa es un acrilato. 2- Device according to claim 1, CHARACTERIZED in that the aqueous based adhesive is an acrylate. 3- Dispositivo de acuerdo a la reivindicación 1, CARACTERIZADO porque los sustratos (1) y (3) son láminas poliméricas. 3- Device according to claim 1, CHARACTERIZED because the substrates (1) and (3) are polymeric sheets. 4- Dispositivo de acuerdo a las reivindicaciones anteriores, CARACTERIZADOS porque el sustrato (1) es de color blanco, mientras que el sustrato (3) es transparente. 4- Device according to the preceding claims, CHARACTERIZED because the substrate (1) is white, while the substrate (3) is transparent. 5- Dispositivo de acuerdo a la reivindicación anterior, CARACTERIZADO porque la capa de adhesivo tiene un espesor no mayor de 40 micrones. 5- Device according to the preceding claim, CHARACTERIZED because the adhesive layer has a thickness not greater than 40 microns. 6- Dispositivo de acuerdo a las reivindicaciones anteriores, CARACTERIZADO porque el espesor de los sustratos (1) y (3) es mayor que el espesor de la capa de adhesivo o acrilato. 6- Device according to the preceding claims, CHARACTERIZED because the thickness of the substrates (1) and (3) is greater than the thickness of the adhesive or acrylate layer. 7- Dispositivo de acuerdo a las reivindicaciones, CARACTERIZADO porque los sustratos tienen un espesor no mayor a 60 micrones. 7- Device according to the claims, CHARACTERIZED because the substrates have a thickness not exceeding 60 microns. 8- Procedimiento para la determinación de identidades y patologías moleculares a partir del ADN de células epiteliales retenidas en el dispositivo de la reivindicación 1, CARACTERIZADO porque comprende tomar una muestra genética dactilar, palmar o plantal, por medio del dispositivo de toma de muestra; y extraer el material genético de dicho dispositivo por ¡sopado, utilizando un KIT de reactivos. 8- Procedure for the determination of molecular identities and pathologies from the DNA of epithelial cells retained in the device of claim 1, CHARACTERIZED because it comprises taking a fingerprint, palmar or plantal genetic sample, by means of the sampling device; and extract the genetic material from said device by means of soups, using a reagent KIT.
PCT/CL2017/000004 2017-02-15 2017-02-15 Non-invasive device for taking genetic samples Ceased WO2018148853A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CL2017/000004 WO2018148853A1 (en) 2017-02-15 2017-02-15 Non-invasive device for taking genetic samples

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CL2017/000004 WO2018148853A1 (en) 2017-02-15 2017-02-15 Non-invasive device for taking genetic samples

Publications (1)

Publication Number Publication Date
WO2018148853A1 true WO2018148853A1 (en) 2018-08-23

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PCT/CL2017/000004 Ceased WO2018148853A1 (en) 2017-02-15 2017-02-15 Non-invasive device for taking genetic samples

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175162A1 (en) * 2000-06-20 2003-09-18 Takanori Anazawa Microdevice having multilayer structure and method for fabricating the same
US6659038B2 (en) * 2000-09-26 2003-12-09 Duwimax International S.A. Safety identification device
WO2008082621A1 (en) * 2007-01-02 2008-07-10 Idea Inc Non-intrusive security seal kit with stamping to obtain dna and genetic patterns of people
US7456025B2 (en) * 2001-08-28 2008-11-25 Porex Corporation Sintered polymer membrane for analyte detection device
WO2016016335A1 (en) * 2014-07-30 2016-02-04 Tracesa Limited Fluid identification system
US9365753B2 (en) * 2008-12-30 2016-06-14 Sicpa Holding Sa Acrylic adhesive for assembling elements contacting biological substances

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030175162A1 (en) * 2000-06-20 2003-09-18 Takanori Anazawa Microdevice having multilayer structure and method for fabricating the same
US6659038B2 (en) * 2000-09-26 2003-12-09 Duwimax International S.A. Safety identification device
US7456025B2 (en) * 2001-08-28 2008-11-25 Porex Corporation Sintered polymer membrane for analyte detection device
WO2008082621A1 (en) * 2007-01-02 2008-07-10 Idea Inc Non-intrusive security seal kit with stamping to obtain dna and genetic patterns of people
US9365753B2 (en) * 2008-12-30 2016-06-14 Sicpa Holding Sa Acrylic adhesive for assembling elements contacting biological substances
WO2016016335A1 (en) * 2014-07-30 2016-02-04 Tracesa Limited Fluid identification system

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
JIANG, G. ET AL.: "DNA/PEI/Alginate Polyplex as an Efficient In Vivo Gene Delivery System", BIOTECHNOLOGY AND BIOPROCESS ENGINEERING, vol. 12, December 2007 (2007-12-01), pages 684 - 689, XP055535507 *

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