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WO2001077350A3 - Compositions and methods for inhibiting gene expression - Google Patents

Compositions and methods for inhibiting gene expression Download PDF

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Publication number
WO2001077350A3
WO2001077350A3 PCT/US2001/011436 US0111436W WO0177350A3 WO 2001077350 A3 WO2001077350 A3 WO 2001077350A3 US 0111436 W US0111436 W US 0111436W WO 0177350 A3 WO0177350 A3 WO 0177350A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
present
eukaryotic cell
endogenous gene
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/011436
Other languages
French (fr)
Other versions
WO2001077350A2 (en
Inventor
Kenneth E Palmer
Gregory P Pogue
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Large Scale Biology Corp
Original Assignee
Large Scale Biology Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp filed Critical Large Scale Biology Corp
Priority to AU2001253255A priority Critical patent/AU2001253255A1/en
Publication of WO2001077350A2 publication Critical patent/WO2001077350A2/en
Publication of WO2001077350A3 publication Critical patent/WO2001077350A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention provides an eukaryotic recombinant vector suited for bi-directional transcription of a transgene to yield both sense and antisense RNA transcripts of the transgene in an eukaryotic cell. The invention vectors are particularly suited for mediating gene silencing in a variety of biological systems. The present invention also provides host cells and transgenic plants comprising the invention vectors. Further provided by the invention are methods of inhibiting expression of an endogenous gene present in an eukaryotic cell. Also included is a method of identifying a biological function(s) of an endogenous gene of interest in an eukaryotic cell by selectively inhibiting the expression of the endogenous gene.
PCT/US2001/011436 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression Ceased WO2001077350A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001253255A AU2001253255A1 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54557400A 2000-04-07 2000-04-07
US09/545,574 2000-04-07

Publications (2)

Publication Number Publication Date
WO2001077350A2 WO2001077350A2 (en) 2001-10-18
WO2001077350A3 true WO2001077350A3 (en) 2002-08-08

Family

ID=24176765

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/011436 Ceased WO2001077350A2 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Country Status (2)

Country Link
AU (1) AU2001253255A1 (en)
WO (1) WO2001077350A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002059257A2 (en) * 2000-10-31 2002-08-01 Commonwealth Scientific And Industrial Research Organisation Method and means for producing barley yellow dwarf virus resistant cereal plants
WO2002100199A2 (en) 2001-06-08 2002-12-19 Vector Tobacco Ltd. Modifying nicotine and nitrosamine levels in tobacco
EP1575976A4 (en) 2001-11-02 2006-08-23 Insert Therapeutics Inc Methods and compositions for therapeutic use of rna interference
CA2525976A1 (en) 2002-05-23 2003-12-04 Ceptyr, Inc. Modulation of ptp1b signal transduction by rna interference
US7148342B2 (en) 2002-07-24 2006-12-12 The Trustees Of The University Of Pennyslvania Compositions and methods for sirna inhibition of angiogenesis
AU2002951347A0 (en) * 2002-09-09 2002-09-26 Benitec Australia Ltd Genetic silencing
JP2006522163A (en) 2003-04-07 2006-09-28 グリコジェネシス， インク． Compositions and uses of galectin antagonists
WO2005097207A2 (en) 2004-03-26 2005-10-20 Curis, Inc. Rna interference modulators of hedgehog signaling and uses thereof
KR100895749B1 (en) 2004-04-05 2009-04-30 더 리전트 오브 더 유니버시티 오브 캘리포니아 Modulation of nkg2d
JP2008506703A (en) 2004-07-14 2008-03-06 ユニバーシティ オブ ユタ リサーチ ファウンデーション Netrin-related compounds and uses
US20060063208A1 (en) 2004-08-02 2006-03-23 Woolf Clifford J DRG11-responsive (DRAGON) gene and uses thereof
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization
WO2006105361A2 (en) 2005-03-31 2006-10-05 Calando Pharmaceuticals, Inc. Inhibitors of ribonucleotide reductase subunit 2 and uses thereof
WO2008127710A2 (en) 2007-04-13 2008-10-23 Dana Farber Cancer Institute Methods for treating cancer resistant to erbb therapeutics
EP2164875A4 (en) 2007-05-24 2011-11-30 Us Dept Veterans Affairs TRANSDUCTION OF INTRANUCLEAR PROTEINS BY RECOVERING NUCLEOSIDES
AU2009232355A1 (en) 2008-04-04 2009-10-08 Calando Pharmaceuticals, Inc. Compositions and use of EPAS1 inhibitors
AU2009322279A1 (en) 2008-12-04 2011-07-14 Opko Pharmaceuticals, Llc Compositions and methods for selective inhibition of pro-angiogenic VEGF isoforms
IT1398977B1 (en) 2009-06-25 2013-03-28 Icgeb ANTI-PANT AGENT SPECIFIC FOR CEREBRAL CANCERS WITH RECQ1 SUPPRESSION MECHANISM
GB201103167D0 (en) 2011-02-24 2011-04-06 Isis Innovation Gene silencing
WO2014052789A1 (en) 2012-09-28 2014-04-03 The University Of North Carolina At Chapel Hill Aav vectors targeted to oligodendrocytes
US10300073B2 (en) 2014-10-14 2019-05-28 The Regents Of The University Of California Use of CDK9 and BRD4 inhibitors to inhibit inflammation
RU2727015C2 (en) 2014-11-21 2020-07-17 Дзе Юниверсити Оф Норт Каролина Эт Чепел Хилл Aav vectors aimed at the central nervous system
AU2015357489A1 (en) 2014-12-05 2017-07-06 Case Western Reserve University Compositions and methods of modulating S-nitrosylation
US10308705B2 (en) 2015-02-06 2019-06-04 The University Of North Carolina At Chapel Hill Optimized human clotting factor VIII gene expression cassettes and their use
WO2016140921A1 (en) 2015-03-02 2016-09-09 180 Therapeutics Lp Method of treating a localized fibrotic disorder using an il-33 antagonist
MA53246A (en) 2016-07-27 2021-07-28 Univ Case Western Reserve COMPOUNDS AND METHODS FOR PROMOTING MYELINATION
ES2968892T3 (en) 2016-12-08 2024-05-14 Univ Case Western Reserve Methods and compositions to increase the production of functional myelin
EP3807297B1 (en) 2018-06-12 2025-10-22 The University of North Carolina at Chapel Hill Synthetic liver-tropic adeno-associated virus capsids and uses thereof
AU2020216480A1 (en) * 2019-01-31 2021-09-09 Oregon Health & Science University Methods for using transcription-dependent directed evolution of AAV capsids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ROGERS S A ET AL: "ANALYSIS OF CHLOROPLAST PROMOTERS USING BIDIRECTIONAL TRANSCRIPTION VECTORS", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 15, no. 3, 1990, pages 421 - 435, XP002159238, ISSN: 0167-4412 *
SPICER D B ET AL: "AN ANTISENSE PROMOTER OF THE MURINE C-MYC GENE IS LOCALIZED WITHIN INTRON 2", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 12, no. 3, March 1992 (1992-03-01), pages 1324 - 1329, XP002910389, ISSN: 0270-7306 *
SUREAU ALAIN ET AL: "Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.", NUCLEIC ACIDS RESEARCH, vol. 25, no. 22, 15 November 1997 (1997-11-15), pages 4513 - 4522, XP002197926, ISSN: 0305-1048 *
VELHAGEN IRIS ET AL: "An antisense promoter within the hepatitis B virus X gene.", INTERVIROLOGY, vol. 38, no. 3-4, 1995, pages 127 - 133, XP008002810, ISSN: 0300-5526 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7304220B2 (en) 1997-06-12 2007-12-04 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression
US6911541B2 (en) 2000-08-30 2005-06-28 North Carolina State University Promoter fragment that is recognized by the product of the tobacco Nic gene
US7192771B2 (en) 2000-08-30 2007-03-20 North Carolina State University Plant promoter sequence
US7189570B2 (en) 2000-11-07 2007-03-13 North Carolina State University Putrescine-n-methyltransferase promoter

Also Published As

Publication number Publication date
WO2001077350A2 (en) 2001-10-18
AU2001253255A1 (en) 2001-10-23

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