[go: up one dir, main page]

WO1993004199A3 - Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids - Google Patents

Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids Download PDF

Info

Publication number
WO1993004199A3
WO1993004199A3 PCT/GB1992/001526 GB9201526W WO9304199A3 WO 1993004199 A3 WO1993004199 A3 WO 1993004199A3 GB 9201526 W GB9201526 W GB 9201526W WO 9304199 A3 WO9304199 A3 WO 9304199A3
Authority
WO
WIPO (PCT)
Prior art keywords
nucleic acids
methods
detecting
quantitating
producing labelled
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/GB1992/001526
Other languages
French (fr)
Other versions
WO1993004199A2 (en
Inventor
Adrian Parton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scientific Generics Ltd
Original Assignee
Scientific Generics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB919117902A external-priority patent/GB9117902D0/en
Priority claimed from GB929202962A external-priority patent/GB9202962D0/en
Application filed by Scientific Generics Ltd filed Critical Scientific Generics Ltd
Publication of WO1993004199A2 publication Critical patent/WO1993004199A2/en
Publication of WO1993004199A3 publication Critical patent/WO1993004199A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Methods for determining the presence or absence of a certain nucleic acid, for producing immobilized nucleic acids and for carrying out cyclic amplification methods like PCR or LCR using immobilized probe-primers are disclosed.
PCT/GB1992/001526 1991-08-20 1992-08-19 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids Ceased WO1993004199A2 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB919117902A GB9117902D0 (en) 1991-08-20 1991-08-20 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
GB9117902.8 1991-08-20
GB9202962.8 1992-02-12
GB929202962A GB9202962D0 (en) 1992-02-12 1992-02-12 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids

Publications (2)

Publication Number Publication Date
WO1993004199A2 WO1993004199A2 (en) 1993-03-04
WO1993004199A3 true WO1993004199A3 (en) 1993-04-15

Family

ID=26299423

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB1992/001526 Ceased WO1993004199A2 (en) 1991-08-20 1992-08-19 Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids

Country Status (1)

Country Link
WO (1) WO1993004199A2 (en)

Families Citing this family (98)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6040138A (en) 1995-09-15 2000-03-21 Affymetrix, Inc. Expression monitoring by hybridization to high density oligonucleotide arrays
GB9127415D0 (en) * 1991-12-24 1992-02-19 Swordfish Int Ltd Solid support bound detection and diagnostic system
WO1994002634A1 (en) * 1992-07-24 1994-02-03 University Of South Australia Amplification and detection process
US6277975B1 (en) 1992-10-23 2001-08-21 Genetics Institute, Inc. Fusions of P-selectin ligand protein and polynucleotides encoding same
GB9301122D0 (en) * 1993-01-21 1993-03-10 Scient Generics Ltd Method of analysis/separation
DE4301693A1 (en) * 1993-01-22 1994-07-28 Cytech Biomedical Inc Amplification methods and methods for the detection of solid phase polynucleotide sequences
GB9306729D0 (en) * 1993-03-31 1993-05-26 British Tech Group Improvements in separators
RU2041262C1 (en) * 1993-08-11 1995-08-09 Институт молекулярной биологии им.В.А.Энгельгардта РАН Method for immobilization of water soluble bioorganic compounds on capillary-porous carrier
CN1152946A (en) * 1994-05-28 1997-06-25 特普尼尔医学有限公司 Producing copies of nucleic acids
GB9415129D0 (en) * 1994-07-27 1994-09-14 Lynxvale Ltd Oligonucleotides and their use
US6060288A (en) * 1994-08-03 2000-05-09 Mosaic Technologies Method for performing amplification of nucleic acid on supports
US5641658A (en) * 1994-08-03 1997-06-24 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid with two primers bound to a single solid support
US6090592A (en) * 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
AT401062B (en) * 1994-09-26 1996-06-25 Immuno Ag Method for quantifying nucleic acids
FR2726286B1 (en) * 1994-10-28 1997-01-17 Genset Sa SOLID PHASE NUCLEIC ACID AMPLIFICATION PROCESS AND REAGENT KIT USEFUL FOR CARRYING OUT SAID PROCESS
US5641634A (en) 1995-11-30 1997-06-24 Mandecki; Wlodek Electronically-indexed solid-phase assay for biomolecules
US6051377A (en) * 1995-11-30 2000-04-18 Pharmaseq, Inc. Multiplex assay for nucleic acids employing transponders
US5736332A (en) * 1995-11-30 1998-04-07 Mandecki; Wlodek Method of determining the sequence of nucleic acids employing solid-phase particles carrying transponders
WO1997019958A1 (en) 1995-11-30 1997-06-05 Wlodek Mandecki Screening of drugs from chemical combinatorial libraries employing transponders
US6001571A (en) 1995-11-30 1999-12-14 Mandecki; Wlodek Multiplex assay for nucleic acids employing transponders
GB2308188A (en) * 1995-12-14 1997-06-18 Tepnel Medical Ltd Assaying immobilised nucleic acid by primer extension
EP0880598A4 (en) * 1996-01-23 2005-02-23 Affymetrix Inc Nucleic acid analysis techniques
CA2244891C (en) * 1996-02-09 2008-12-30 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6852487B1 (en) 1996-02-09 2005-02-08 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
WO1997045559A1 (en) 1996-05-29 1997-12-04 Cornell Research Foundation, Inc. Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
AU6846698A (en) 1997-04-01 1998-10-22 Glaxo Group Limited Method of nucleic acid amplification
US5981166A (en) * 1997-04-23 1999-11-09 Pharmaseq, Inc. Screening of soluble chemical compounds for their pharmacological properties utilizing transponders
WO1999022023A2 (en) * 1997-10-29 1999-05-06 Mira Diagnostica Gmbh Method for identifying micro-organisms
EP1016731A1 (en) * 1998-01-08 2000-07-05 Laboratory of Molecular Biophotonics Probes for detecting target nucleic acid, method of detecting target nucleic acid, and solid phase for detecting target nucleic acid and process for producing the same
AR021833A1 (en) 1998-09-30 2002-08-07 Applied Research Systems METHODS OF AMPLIFICATION AND SEQUENCING OF NUCLEIC ACID
BE1012275A3 (en) * 1998-11-12 2000-08-01 Univ Bruxelles Covalent oligonucleotide fixation to nylon surface
GB9904991D0 (en) * 1999-03-05 1999-04-28 Univ Nottingham Genetic screening
SE9902410D0 (en) 1999-06-24 1999-06-24 Cavidi Tech Ab Reverse transcriptase assay kit, use thereof and method for analysis of RT activity in biological samples
JP4860869B2 (en) * 1999-12-29 2012-01-25 オックスフォード ジーン テクノロジー アイピー リミティド Method for amplifying and detecting a plurality of polynucleotides on a solid support
WO2001079548A2 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
EP1207209A3 (en) * 2000-11-09 2002-09-11 Agilent Technologies, Inc. (a Delaware corporation) Methods using arrays for detection of single nucleotide polymorphisms
US7771975B2 (en) 2001-04-02 2010-08-10 Point-2-Point Genomics Limited Polynucleotide analysis using combinatorial PCR
AR031640A1 (en) 2000-12-08 2003-09-24 Applied Research Systems ISOTHERMAL AMPLIFICATION OF NUCLEIC ACIDS IN A SOLID SUPPORT
DE10160983B4 (en) * 2001-12-05 2004-12-09 Epigenomics Ag Method and integrated device for the detection of cytosine methylation
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
GB0514910D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Method for sequencing a polynucleotide template
EP1930422B1 (en) * 2005-08-19 2013-01-23 Sumitomo Bakelite Company, Ltd. Method for producing cdna and rna chains and nucleotide-immobilized support
GB0522310D0 (en) 2005-11-01 2005-12-07 Solexa Ltd Methods of preparing libraries of template polynucleotides
GB0524069D0 (en) 2005-11-25 2006-01-04 Solexa Ltd Preparation of templates for solid phase amplification
CA2641851A1 (en) 2006-02-08 2007-08-16 Eric Hans Vermaas Method for sequencing a polynucleotide template
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
FI20075124A0 (en) * 2007-02-21 2007-02-21 Valtion Teknillinen Method and test kit for detection of nucleotide variations
US12060554B2 (en) 2008-03-10 2024-08-13 Illumina, Inc. Method for selecting and amplifying polynucleotides
WO2010038042A1 (en) 2008-10-02 2010-04-08 Illumina Cambridge Ltd. Nucleic acid sample enrichment for sequencing applications
LT2669387T (en) 2009-08-25 2016-10-25 Illumina, Inc. Methods for selecting and amplifying polynucleotides
US8182994B2 (en) 2009-09-15 2012-05-22 Illumina Cambridge Limited Centroid markers for image analysis of high denisty clusters in complex polynucleotide sequencing
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
EP3901280B1 (en) 2012-10-17 2025-03-12 10x Genomics Sweden AB Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
GB201301857D0 (en) * 2013-02-01 2013-03-20 Selvi Ozan Method
US9868979B2 (en) 2013-06-25 2018-01-16 Prognosys Biosciences, Inc. Spatially encoded biological assays using a microfluidic device
ES2994053T3 (en) 2015-04-10 2025-01-17 10X Genomics Sweden Ab Spatially distinguished, multiplex nucleic acid analysis of biological specimens
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020123301A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Generating spatial arrays with gradients
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
EP3976820A1 (en) 2019-05-30 2022-04-06 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4025711A2 (en) 2019-11-08 2022-07-13 10X Genomics, Inc. Enhancing specificity of analyte binding
FI3891300T3 (en) 2019-12-23 2023-05-10 10X Genomics Inc Methods for spatial analysis using rna-templated ligation
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) * 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
CN115916999A (en) 2020-04-22 2023-04-04 10X基因组学有限公司 Methods for spatial analysis using targeted RNA depletion
ES2989052T3 (en) 2020-05-22 2024-11-25 10X Genomics Inc Simultaneous spatiotemporal measurement of gene expression and cellular activity
AU2021275906A1 (en) 2020-05-22 2022-12-22 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
EP4162074B1 (en) 2020-06-08 2024-04-24 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
ES2994976T3 (en) 2020-06-25 2025-02-05 10X Genomics Inc Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
EP4121555A1 (en) 2020-12-21 2023-01-25 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022221425A1 (en) 2021-04-14 2022-10-20 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
EP4320271B1 (en) 2021-05-06 2025-03-19 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
ES3030033T3 (en) 2021-06-03 2025-06-26 10X Genomics Inc Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
ES3011462T3 (en) 2021-09-01 2025-04-07 10X Genomics Inc Methods for blocking a capture probe on a spatial array
EP4419707A1 (en) 2021-11-10 2024-08-28 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988001302A1 (en) * 1986-08-11 1988-02-25 Siska Diagnostics, Inc. Nucleic acid probe assay methods and compositions
WO1989011546A1 (en) * 1988-05-24 1989-11-30 Gunnar Paulsen Dna-analysis method involving gene amplification and magnetic particles
WO1990006042A2 (en) * 1988-11-21 1990-06-14 Dynal As Detection and quantitative determination of rna and dna
EP0374665A2 (en) * 1988-12-23 1990-06-27 Miles Inc. Assay of sequences using amplified genes
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988001302A1 (en) * 1986-08-11 1988-02-25 Siska Diagnostics, Inc. Nucleic acid probe assay methods and compositions
WO1989011546A1 (en) * 1988-05-24 1989-11-30 Gunnar Paulsen Dna-analysis method involving gene amplification and magnetic particles
WO1990006042A2 (en) * 1988-11-21 1990-06-14 Dynal As Detection and quantitative determination of rna and dna
EP0374665A2 (en) * 1988-12-23 1990-06-27 Miles Inc. Assay of sequences using amplified genes
WO1990011369A1 (en) * 1989-03-22 1990-10-04 Cemu Bioteknik Ab Solid phase diagnosis of medical conditions
WO1991000868A1 (en) * 1989-07-07 1991-01-24 National Research Development Corporation Immobilised polynucleotides

Also Published As

Publication number Publication date
WO1993004199A2 (en) 1993-03-04

Similar Documents

Publication Publication Date Title
WO1993004199A3 (en) Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
CA2307177A1 (en) Methods for detecting contamination in molecular diagnostics using pcr
WO1993015229A3 (en) Amplification of assay reporters by nucleic acid replication
EP0640691A3 (en) Detection of mycobacteria by multiplex nucleic acid amplification.
AU1502395A (en) Detection of nucleic acid amplification
AU685144B2 (en) Chlamydia trachomatis by polymerase chain reaction using biotin labelled lina primers and capture probes
CA2100159A1 (en) Rapid assays for amplification products
EP0822256A3 (en) Modified thermostable DNA polymerase, and DNA polymerase composition for nucleic acid amplification
EP0756010A3 (en) Method for detecting, identifying, and quantitating organisms and viruses
EP0605003A3 (en) Assay method for detecting the presence of bacteria.
CA2135607A1 (en) Chemical method for the analysis of dna sequences
EP0599338A3 (en) Method for detecting target nucleic acid.
CA2172248A1 (en) Self-sustained sequence replication electrochemiluminescent nucleic acid assay
IL111831A (en) Method of detecting the presence of a nucleic acid in a sample
WO1999023249A3 (en) Specific and sensitive method for detecting nucleic acids
ZA862334B (en) Process for amplifying,detecting and/or cloning nucleic acid sequences
WO1997007235A3 (en) All-in-one nucleic acid amplification assay
EP0682121A4 (en) Method of amplifying and detecting target nucleic acid sequence by using thermostable enzymes.
AU6972287A (en) Nucleic acid hybridization assay for microorganisms
WO2002024944A3 (en) Method for detecting at least one nucleic acid sequence
AU3081495A (en) Nucleic acid amplification method using a modified nucleoside, and detection of the amplification product using antibodies
WO1995031571A3 (en) Materials and methods for the detection of mycobacteria
WO1998035058A3 (en) Improved process for detection and quantification of nucleic acid molecules
EP0922771A3 (en) Method for the detection of large concentrations of a virus in blood plasma and/ or blood serum using the polymerase chain reaction
EP1138785B8 (en) Nucleic acid fragment primer or probe, and method of detecting polyhydroxyalkanoate synthesizing microorganism by using the same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL SE

AK Designated states

Kind code of ref document: A3

Designated state(s): JP US

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH DE DK ES FR GB GR IE IT LU MC NL SE

122 Ep: pct application non-entry in european phase