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RU2000116481A - MUTANT N-ACETYL GLUTAMATE SYNTHESIS, A BACTERIA BELONGING TO THE GENUS ESCHERICHIA, METHOD FOR PRODUCING L-ARGININE - Google Patents

MUTANT N-ACETYL GLUTAMATE SYNTHESIS, A BACTERIA BELONGING TO THE GENUS ESCHERICHIA, METHOD FOR PRODUCING L-ARGININE

Info

Publication number
RU2000116481A
RU2000116481A RU2000116481/13A RU2000116481A RU2000116481A RU 2000116481 A RU2000116481 A RU 2000116481A RU 2000116481/13 A RU2000116481/13 A RU 2000116481/13A RU 2000116481 A RU2000116481 A RU 2000116481A RU 2000116481 A RU2000116481 A RU 2000116481A
Authority
RU
Russia
Prior art keywords
arginine
mutant
synthase
producing
acetylglutamate synthase
Prior art date
Application number
RU2000116481/13A
Other languages
Russian (ru)
Inventor
Леонид Романович Птицын
Ирина Борисовна Альтман
Сергей Васильевич Смирнов
Юлия Георгиевна Ростова
Татьяна Абрамовна Ямпольская
Татьяна Викторовна Леонова
Михаил Маркович Гусятинер
Original Assignee
Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
Filing date
Publication date
Application filed by Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" filed Critical Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика"
Priority to JP2001180219A priority Critical patent/JP4682454B2/en
Priority to ES01114572T priority patent/ES2228699T3/en
Priority to EP01114572A priority patent/EP1170361B1/en
Priority to DE60106526T priority patent/DE60106526T2/en
Priority to US09/886,135 priority patent/US6790647B2/en
Priority to CNB011259191A priority patent/CN1180082C/en
Publication of RU2000116481A publication Critical patent/RU2000116481A/en
Priority to US10/891,122 priority patent/US7169586B2/en

Links

Claims (6)

1. Мутантная N-ацетилглутамат синтаза, отличающаяся тем, что последовательность аминокислот, соответствующая положениям с 15 по 19 в природной N-ацетилгутамат синтазе, заменена любой из последовательностей аминокислот под номерами 1-4 (SЕО ID NOS: 1 - 4) и ингибирование L-аргинином но типу обратной связи утрачено.1. Mutant N-acetylglutamate synthase, characterized in that the amino acid sequence corresponding to positions 15 to 19 in the natural N-acetylgutamate synthase is replaced by any of the amino acid sequences numbered 1-4 (SEO ID NOS: 1-4) and the inhibition of L -arginine but the type of feedback is lost. 2. Мутантная N-ацетилглутамат синтаза по п. 1, отличающаяся тем. что природной N-ацетилглутамат синтазой является N-ацетилглутамат синтаза из Еscherichia coli. 2. The mutant N-acetylglutamate synthase according to claim 1, characterized in that. that the natural N-acetylglutamate synthase is N-acetylglutamate synthase from Escherichia coli. 3. Мутантная N-ацетилглутамат синтаза по п. 1, отличающаяся тем, что такая синтаза содержит делеции, замены, вставки или добавления одной или нескольких аминокислот в одном или множестве положений, кроме положений с 15 по 19, и ингибирование L-аргинином такой синтазы по типу обратной связи утрачено. 3. The mutant N-acetylglutamate synthase according to claim 1, characterized in that such synthase contains deletions, substitutions, insertions or additions of one or more amino acids in one or many positions, except positions 15 to 19, and the inhibition of such synthase by L-arginine feedback type lost. 4. ДНК, кодирующая мутантную N-ацетилглутамат синтазу по любому из пп. 1-3. 4. DNA encoding the mutant N-acetylglutamate synthase according to any one of paragraphs. 1-3. 5. Бактерия, принадлежащая к роду Escherichia, отличающаяся тем, что такая бактерия трансформирована ДНК по пункту 4 и обладает активностью по продукции L-аргинина. 5. A bacterium belonging to the genus Escherichia, characterized in that such a bacterium is transformed with DNA according to paragraph 4 and is active in the production of L-arginine. 6. Способ продуцирования L-аргинина, включающий стадии выращивания бактерии по пункту 5 в питательной среде для продукции и накопления L-аргинина в питательной среде и сбора L-аргинина из культуральной жидкости. 6. A method for producing L-arginine, comprising the steps of growing the bacterium according to paragraph 5 in a culture medium for producing and storing L-arginine in a culture medium and collecting L-arginine from the culture fluid.
RU2000116481/13A 2000-06-28 2000-06-28 MUTANT N-ACETYL GLUTAMATE SYNTHESIS, A BACTERIA BELONGING TO THE GENUS ESCHERICHIA, METHOD FOR PRODUCING L-ARGININE RU2000116481A (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
JP2001180219A JP4682454B2 (en) 2000-06-28 2001-06-14 Process for producing novel mutant N-acetylglutamate synthase and L-arginine
ES01114572T ES2228699T3 (en) 2000-06-28 2001-06-18 NEW MUTANT OF N-ACETIGLTAMATE SYNTHEASE AND PROCEDURE FOR THE PRODUCTION OF ARGININE.
EP01114572A EP1170361B1 (en) 2000-06-28 2001-06-18 New mutant N-Acetylglutamate synthase and method for L-Arginine production
DE60106526T DE60106526T2 (en) 2000-06-28 2001-06-18 New N-acetylglutamate synthase mutant and a process for the production of L-arginine
US09/886,135 US6790647B2 (en) 2000-06-28 2001-06-22 Mutant N-acetylglutamate synthase and method for L-arginine production
CNB011259191A CN1180082C (en) 2000-06-28 2001-06-28 Production method of novel mutant N-acetylglutamate synthetase and L-arginine
US10/891,122 US7169586B2 (en) 2000-06-28 2004-07-15 Mutant N-acetylglutamate synthase and method for L-arginine production

Related Child Applications (1)

Application Number Title Priority Date Filing Date
RU2001112869/13A Substitution RU2215783C2 (en) 2000-06-28 2001-05-15 Mutant n-acetylglutamate synthase (variants), dna fragment, strain of microorganism escherichia coli as producer of arginine and method for preparing l-arginine

Publications (1)

Publication Number Publication Date
RU2000116481A true RU2000116481A (en) 2002-07-10

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