GB1478460A - Method of preparing glucopeptides - Google Patents
Method of preparing glucopeptidesInfo
- Publication number
- GB1478460A GB1478460A GB4061273A GB4061273A GB1478460A GB 1478460 A GB1478460 A GB 1478460A GB 4061273 A GB4061273 A GB 4061273A GB 4061273 A GB4061273 A GB 4061273A GB 1478460 A GB1478460 A GB 1478460A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glucopeptide
- centrifuging
- crude
- neutralization
- purified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 abstract 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 3
- 238000001914 filtration Methods 0.000 abstract 3
- 238000006386 neutralization reaction Methods 0.000 abstract 3
- 239000002244 precipitate Substances 0.000 abstract 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 2
- 239000002245 particle Substances 0.000 abstract 2
- 230000001376 precipitating effect Effects 0.000 abstract 2
- 239000011780 sodium chloride Substances 0.000 abstract 2
- 239000006228 supernatant Substances 0.000 abstract 2
- 241000283690 Bos taurus Species 0.000 abstract 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 abstract 1
- 239000005909 Kieselgur Substances 0.000 abstract 1
- 241001465754 Metazoa Species 0.000 abstract 1
- 108090000526 Papain Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 1
- 239000003513 alkali Substances 0.000 abstract 1
- 239000012736 aqueous medium Substances 0.000 abstract 1
- 229960002798 cetrimide Drugs 0.000 abstract 1
- YMKDRGPMQRFJGP-UHFFFAOYSA-M cetylpyridinium chloride Chemical compound [Cl-].CCCCCCCCCCCCCCCC[N+]1=CC=CC=C1 YMKDRGPMQRFJGP-UHFFFAOYSA-M 0.000 abstract 1
- 229960001927 cetylpyridinium chloride Drugs 0.000 abstract 1
- 238000001035 drying Methods 0.000 abstract 1
- 210000001198 duodenum Anatomy 0.000 abstract 1
- 229940088598 enzyme Drugs 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 230000003301 hydrolyzing effect Effects 0.000 abstract 1
- 230000000055 hyoplipidemic effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 229940055729 papain Drugs 0.000 abstract 1
- 235000019834 papain Nutrition 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 abstract 1
- 229960004319 trichloroacetic acid Drugs 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/37—Digestive system
- A61K35/38—Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Nutrition Science (AREA)
- Physiology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Developmental Biology & Embryology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
1478460 Glucopeptides E FISHER J L J SZABO and N SZAPANOS 19 Aug 1974 [29 Aug 1973] 40612/73 Heading C3H Hypolipaemic glucopeptides are prepared by freeing bovine duodenum material in small particles from fat, hydrolysing the animal tissue in an aqueous medium which contains an alkali or an enzyme, e.g. papain, separating undispersed particles by filtration or centrifuging, precipitating crude glucopeptide from the residual liquid by addition of alcohol and then purifying the crude glucopeptide. The alkaline medium may be sodium hydroxide of IN concentration and hydrolysis is continued for 4 days at room temperature, followed by neutralization to a pH from 6À5 to 7À5. Hydrochloric acid may be used for neutralization to a pH of from 7 to 7À5 or acetic acid may be used for neutralization to a pH of from 6À5 to 7. The crude precipitated glucopeptide may be dissolved in sodium chloride solution, the solution clarified by centrifuging, a precipitant then being added to the supernatant, removing the resultant precipitate, dissolving the precipitate in sodium chloride solution, clarifying the solution by centrifuging, adding alcohol to the supernatant and separating the final precipitate by filtering or centrifuging. The precipitant may be Cetrimide, trichloracetic acid or cetyl pyridinium chloride. Preferably the crude glucopeptide is purified by drying, mixing and pulverizing with cellulose powder or diatomaceous earth, charging a chromatographic column with the mixture, passing a solvent through the column, eluting the glucopeptide with a phosphate buffer at pH 7À8 and precipitating the glucopeptide with two volumes of alcohol, and filtering the purified glucopeptide. The purified glucopeptide may be dried, ground and screened.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4061273A GB1478460A (en) | 1974-08-19 | 1974-08-19 | Method of preparing glucopeptides |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4061273A GB1478460A (en) | 1974-08-19 | 1974-08-19 | Method of preparing glucopeptides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1478460A true GB1478460A (en) | 1977-06-29 |
Family
ID=10415755
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB4061273A Expired GB1478460A (en) | 1974-08-19 | 1974-08-19 | Method of preparing glucopeptides |
Country Status (1)
| Country | Link |
|---|---|
| GB (1) | GB1478460A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109393458A (en) * | 2018-10-16 | 2019-03-01 | 宁夏沃福百瑞枸杞产业股份有限公司 | A kind of preparation method of Lycium chinense glycopeptide particle electuary |
-
1974
- 1974-08-19 GB GB4061273A patent/GB1478460A/en not_active Expired
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109393458A (en) * | 2018-10-16 | 2019-03-01 | 宁夏沃福百瑞枸杞产业股份有限公司 | A kind of preparation method of Lycium chinense glycopeptide particle electuary |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PS | Patent sealed | ||
| PCNP | Patent ceased through non-payment of renewal fee |