AR020075A1 - Metodo y construccion para preparar una planta transgenica. - Google Patents
Metodo y construccion para preparar una planta transgenica.Info
- Publication number
- AR020075A1 AR020075A1 ARP990102315A ARP990102315A AR020075A1 AR 020075 A1 AR020075 A1 AR 020075A1 AR P990102315 A ARP990102315 A AR P990102315A AR P990102315 A ARP990102315 A AR P990102315A AR 020075 A1 AR020075 A1 AR 020075A1
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- gene
- coix
- transgenic
- transgenic plant
- Prior art date
Links
- 230000009261 transgenic effect Effects 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 5
- 238000010276 construction Methods 0.000 title abstract 2
- 241000196324 Embryophyta Species 0.000 abstract 11
- 108090000623 proteins and genes Proteins 0.000 abstract 7
- 241000209205 Coix Species 0.000 abstract 5
- 240000008042 Zea mays Species 0.000 abstract 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract 2
- 235000005822 corn Nutrition 0.000 abstract 2
- 230000030279 gene silencing Effects 0.000 abstract 2
- 240000005979 Hordeum vulgare Species 0.000 abstract 1
- 235000007340 Hordeum vulgare Nutrition 0.000 abstract 1
- 241000209510 Liliopsida Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract 1
- 241000209056 Secale Species 0.000 abstract 1
- 235000007238 Secale cereale Nutrition 0.000 abstract 1
- 240000006394 Sorghum bicolor Species 0.000 abstract 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 abstract 1
- 235000021307 Triticum Nutrition 0.000 abstract 1
- 244000098338 Triticum aestivum Species 0.000 abstract 1
- 238000012226 gene silencing method Methods 0.000 abstract 1
- 244000045947 parasite Species 0.000 abstract 1
- 230000001172 regenerating effect Effects 0.000 abstract 1
- 235000009566 rice Nutrition 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
- 238000012250 transgenic expression Methods 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Botany (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Pharmacology & Pharmacy (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Un método para preparar una planta transgénica monocotiledonea distinta de coix que exprese un gen seleccionado que comprende los parsos de: (a)proveer ungen seleccionado; (b)preparar una construccion que contenga dicho gen ligado operativamente aun promotor Coix; (c)transformar una célula receptora de unaplanta monocotiledonea distinta de Coix con dicha construccion; y (d)regenerar una planta transgénica monocotiledonea que exprese dicho gen de dicha célulareceptora. La planta transgénicamono cotiledonea es una planta seleccionada del grupo formado por arroz, trigo, cebada, centeno, sorgo, y maíz y el gen puedeser potencialmente cualquier gen que se quiera expresar en plantas transgénicas. Se provee también un método para criarplantas qu e comprende los pasos de:(a)obtener una planta progenie de cualquier generacion de una planta transgenica monocotiledonea preparada de acuerdo con los métodos descriptos anteriormente,donde la planta progenie comprende dicha construccion;y (b)cruzar la planta consigo misma o con una segunda planta. Con este metodo, se evita elsilenciamiento de genes mediante el uso de secuencias homologas de plantas monocotiledoneas del género Coix para la transformacion. En estas secuenciasdetransgenes se inc luyen promotores, potenciales, secuencias de codificacion y terminadores de Coix. Es conveniente contar con alternativas de transgenesderivados de maíz para su expresion en maíz por cuanto el silenciamiento de genes basado enhomologías puede limita r o eliminar de forma efectiva la expresiontransgénica.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/078,972 US6635806B1 (en) | 1998-05-14 | 1998-05-14 | Methods and compositions for expression of transgenes in plants |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR020075A1 true AR020075A1 (es) | 2002-04-10 |
Family
ID=22147323
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP990102315A AR020075A1 (es) | 1998-05-14 | 1999-05-14 | Metodo y construccion para preparar una planta transgenica. |
| ARP090102514A AR072694A2 (es) | 1998-05-14 | 2009-07-03 | Planta transgenica fertil |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP090102514A AR072694A2 (es) | 1998-05-14 | 2009-07-03 | Planta transgenica fertil |
Country Status (21)
| Country | Link |
|---|---|
| US (5) | US6635806B1 (es) |
| EP (1) | EP1076706B1 (es) |
| JP (2) | JP2002533057A (es) |
| KR (1) | KR20010052350A (es) |
| CN (1) | CN1310760A (es) |
| AR (2) | AR020075A1 (es) |
| AT (1) | ATE385518T1 (es) |
| AU (1) | AU3995799A (es) |
| BR (1) | BR9910455A (es) |
| CA (1) | CA2328129C (es) |
| DE (1) | DE69938101T2 (es) |
| DK (1) | DK1076706T3 (es) |
| ES (1) | ES2301239T3 (es) |
| ID (1) | ID27936A (es) |
| IL (1) | IL139568A0 (es) |
| IN (2) | IN2000DE00321A (es) |
| MX (1) | MXPA00011199A (es) |
| PT (1) | PT1076706E (es) |
| TR (1) | TR200100104T2 (es) |
| WO (1) | WO1999058659A2 (es) |
| ZA (1) | ZA200006576B (es) |
Families Citing this family (92)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6635806B1 (en) * | 1998-05-14 | 2003-10-21 | Dekalb Genetics Corporation | Methods and compositions for expression of transgenes in plants |
| US20040266673A1 (en) * | 2002-07-31 | 2004-12-30 | Peter Bakis | Long lasting natriuretic peptide derivatives |
| US20030172403A1 (en) | 2000-05-02 | 2003-09-11 | Ning Huang | Plant transcription factors and enhanced gene expression |
| US6991824B2 (en) * | 2000-05-02 | 2006-01-31 | Ventria Bioscience | Expression of human milk proteins in transgenic plants |
| US20080050503A1 (en) * | 2000-05-02 | 2008-02-28 | Ning Huang | Expression of human milk proteins in transgenic plants |
| US7417178B2 (en) * | 2000-05-02 | 2008-08-26 | Ventria Bioscience | Expression of human milk proteins in transgenic plants |
| WO2002029070A2 (en) * | 2000-10-05 | 2002-04-11 | The Regents Of The University Of California | Genetic functions required for gene silencing in maize |
| US7214786B2 (en) * | 2000-12-14 | 2007-05-08 | Kovalic David K | Nucleic acid molecules and other molecules associated with plants and uses thereof for plant improvement |
| CA2435972C (en) * | 2001-01-26 | 2011-09-13 | University Of Lausanne | Matrix attachment regions and methods for use thereof |
| EP1466973A4 (en) * | 2001-12-20 | 2006-08-09 | Univ Okayama Nat Univ Corp | PLANT SEQUENCES OF CHARACTERISTIC BASE SEQUENCES AND METHOD OF USE THEREOF |
| KR101229989B1 (ko) | 2003-10-24 | 2013-02-06 | 셀렉시스 에스. 에이. | Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
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| US7855323B2 (en) | 2004-02-10 | 2010-12-21 | Monsanto Technology Llc | Recombinant DNA for gene suppression |
| US20060075522A1 (en) | 2004-07-31 | 2006-04-06 | Jaclyn Cleveland | Genes and uses for plant improvement |
| AR056290A1 (es) | 2005-03-31 | 2007-10-03 | Nippon Soda Co | Metodo para inhibir la produccion de de micotoxina |
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| WO2007081872A2 (en) * | 2006-01-05 | 2007-07-19 | The Curators Of The University Of Missouri | Categorically ranking animals for feed efficiency |
| US8420890B2 (en) | 2006-03-28 | 2013-04-16 | Cornell Research Foundation, Inc. | Use of NAP gene to manipulate leaf senescence in plants |
| US8076536B2 (en) | 2006-05-12 | 2011-12-13 | Monsanto Technology Llc | Methods and compositions for obtaining marker-free transgenic plants |
| EP3608413B1 (en) | 2006-05-16 | 2023-03-15 | Monsanto Technology LLC | Use of non-agrobacterium bacterial species for plant transformation |
| US7855326B2 (en) | 2006-06-06 | 2010-12-21 | Monsanto Technology Llc | Methods for weed control using plants having dicamba-degrading enzymatic activity |
| US8946511B2 (en) * | 2006-10-12 | 2015-02-03 | Monsanto Technology Llc | Plant microRNAs and methods of use thereof |
| EA020590B1 (ru) | 2006-10-16 | 2014-12-30 | Монсанто Текнолоджи Ллс | Способы улучшения жизнеспособности растений |
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| EP3737747A1 (en) | 2018-01-12 | 2020-11-18 | The Texas A&M University System | Increasing plant bioproduct yield |
| CN109169280B (zh) * | 2018-09-20 | 2022-02-08 | 四川农业大学 | 薏苡再生植株的制备方法及所用培养基 |
| CN109593778A (zh) * | 2018-11-08 | 2019-04-09 | 浙江大学 | 一种植物人工智能雄性不育系及应用 |
| CA3130789A1 (en) | 2019-03-07 | 2020-09-10 | The Regents Of The University Of California | Crispr-cas effector polypeptides and methods of use thereof |
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| CN111363775B (zh) * | 2020-03-18 | 2022-08-05 | 浙江工业大学 | 一种生物酶法去消旋化制备l-草铵膦的方法、草铵膦脱氢酶突变体及应用 |
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| CN119842794A (zh) | 2020-09-10 | 2025-04-18 | 孟山都技术公司 | 利用减数分裂和种系启动子增加基因编辑和定点整合事件 |
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| CN113151318B (zh) * | 2021-03-17 | 2022-08-16 | 云南中烟工业有限责任公司 | 一种烟草淀粉分支酶基因NtGBE1及其应用 |
| AU2023302130A1 (en) | 2022-07-05 | 2025-01-09 | University Of Freiburg | Plant regulatory elements and uses thereof |
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| US7705215B1 (en) | 1990-04-17 | 2010-04-27 | Dekalb Genetics Corporation | Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof |
| US5484956A (en) * | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
| US5614393A (en) * | 1991-10-10 | 1997-03-25 | Rhone-Poulenc Agrochimie | Production of γ-linolenic acid by a Δ6-desaturase |
| EP0633940B1 (en) * | 1992-04-02 | 2002-12-04 | SemBioSys Genetics Inc. | Oil-body protein cis-elements as regulatory signals |
| US6326527B1 (en) * | 1993-08-25 | 2001-12-04 | Dekalb Genetics Corporation | Method for altering the nutritional content of plant seed |
| US5716837A (en) | 1995-02-10 | 1998-02-10 | Monsanto Company | Expression of sucrose phosphorylase in plants |
| GB9611981D0 (en) | 1996-06-07 | 1996-08-07 | Zeneca Ltd | Enhancement of gene expression |
| US6635806B1 (en) * | 1998-05-14 | 2003-10-21 | Dekalb Genetics Corporation | Methods and compositions for expression of transgenes in plants |
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