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MX2018006709A - Mutagénesis dirigida de ácidos nucleicos tipo enzima del puente a berberina de tabaco. - Google Patents

Mutagénesis dirigida de ácidos nucleicos tipo enzima del puente a berberina de tabaco.

Info

Publication number
MX2018006709A
MX2018006709A MX2018006709A MX2018006709A MX2018006709A MX 2018006709 A MX2018006709 A MX 2018006709A MX 2018006709 A MX2018006709 A MX 2018006709A MX 2018006709 A MX2018006709 A MX 2018006709A MX 2018006709 A MX2018006709 A MX 2018006709A
Authority
MX
Mexico
Prior art keywords
tobacco
nucleic acids
bridge
directed mutagenesis
acids type
Prior art date
Application number
MX2018006709A
Other languages
English (en)
Other versions
MX388423B (es
Inventor
E Dewey Ralph
S Lewis Ramsey
Original Assignee
Univ North Carolina State
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ North Carolina State filed Critical Univ North Carolina State
Publication of MX2018006709A publication Critical patent/MX2018006709A/es
Publication of MX388423B publication Critical patent/MX388423B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/12—Leaves
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/82—Solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
    • A01H6/823—Nicotiana, e.g. tobacco
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
    • A24B3/00—Preparing tobacco in the factory
    • A24B3/14—Forming reconstituted tobacco products, e.g. wrapper materials, sheets, imitation leaves, rods, cakes; Forms of such products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • A—HUMAN NECESSITIES
    • A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
    • A24D—CIGARS; CIGARETTES; TOBACCO SMOKE FILTERS; MOUTHPIECES FOR CIGARS OR CIGARETTES; MANUFACTURE OF TOBACCO SMOKE FILTERS OR MOUTHPIECES
    • A24D1/00—Cigars; Cigarettes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Manufacture Of Tobacco Products (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención está dirigida a modificaciones de los ácidos nucleicos tipo enzima del puente a berberina y su uso en la modulación de la biosíntesis de nicotina en plantas.
MX2018006709A 2015-12-04 2016-12-02 Mutagénesis dirigida de ácidos nucleicos tipo enzima del puente a berberina de tabaco. MX388423B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562263151P 2015-12-04 2015-12-04
PCT/US2016/064758 WO2017096254A1 (en) 2015-12-04 2016-12-02 Targeted mutagenesis of tobacco berberine bridge enzyme-like nucleic acids

Publications (2)

Publication Number Publication Date
MX2018006709A true MX2018006709A (es) 2019-01-31
MX388423B MX388423B (es) 2025-03-19

Family

ID=58797873

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2018006709A MX388423B (es) 2015-12-04 2016-12-02 Mutagénesis dirigida de ácidos nucleicos tipo enzima del puente a berberina de tabaco.
MX2021014746A MX2021014746A (es) 2015-12-04 2018-05-31 Mutagenesis dirigida de acidos nucleicos tipo enzima del puente a berberina de tabaco.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2021014746A MX2021014746A (es) 2015-12-04 2018-05-31 Mutagenesis dirigida de acidos nucleicos tipo enzima del puente a berberina de tabaco.

Country Status (14)

Country Link
US (5) US10590429B2 (es)
EP (1) EP3383170A4 (es)
JP (3) JP6956084B2 (es)
KR (1) KR20180085794A (es)
CN (2) CN108463103B (es)
AU (2) AU2016362578B2 (es)
BR (1) BR112018011050A2 (es)
CA (1) CA3006996A1 (es)
MA (1) MA43388A (es)
MX (2) MX388423B (es)
MY (1) MY198819A (es)
PH (1) PH12018501162A1 (es)
RU (1) RU2018123563A (es)
WO (1) WO2017096254A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10405571B2 (en) 2015-06-26 2019-09-10 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
BR112018011050A2 (pt) * 2015-12-04 2018-11-21 North Carolina State University mutagênese direcionada de ácidos nucleicos semelhantes à enzima com ligação de ponte de berberina do tabaco
US20200035118A1 (en) 2018-07-27 2020-01-30 Joseph Pandolfino Methods and products to facilitate smokers switching to a tobacco heating product or e-cigarettes
US10897925B2 (en) 2018-07-27 2021-01-26 Joseph Pandolfino Articles and formulations for smoking products and vaporizers
WO2021142296A1 (en) * 2020-01-08 2021-07-15 North Carolina State University A genetic approach for achieving ultra low nicotine content in tobacco
CN115485386B (zh) * 2020-04-17 2024-03-29 日本烟草产业株式会社 低生物碱含量的烟草属植物体及其制备方法
CN114410677A (zh) * 2021-11-22 2022-04-29 云南中烟工业有限责任公司 一种利用CRISPR/Cas9敲除NtBBLs创制低烟碱烟草突变体的方法及应用
US20250051266A1 (en) * 2021-12-23 2025-02-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Novel GLV-Phenolamide: Biosynthesis and Function in Protecting Plants from Herbivore Attack
WO2023141481A2 (en) * 2022-01-20 2023-07-27 North Carolina State University Methods for producing low-nicotine tobacco

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2992898A1 (en) * 2005-02-28 2006-10-19 22Nd Century Limited, Llc Reducing levels of nicotinic alkaloids in plants
US7665472B2 (en) * 2007-11-06 2010-02-23 Alliance One International, Inc. Tobacco cultivar AOB 175 and products therefrom
RU2403831C1 (ru) 2009-07-27 2010-11-20 Государственное научное учреждение Всероссийский научно-исследовательский институт табака, махорки и табачных изделий Российской академии сельскохозяйственных наук (ГНУ ВНИИТТИ Россельхозакадемии) Способ производства бездымного табачного изделия (снюса) (варианты)
BR112018011050A2 (pt) * 2015-12-04 2018-11-21 North Carolina State University mutagênese direcionada de ácidos nucleicos semelhantes à enzima com ligação de ponte de berberina do tabaco

Also Published As

Publication number Publication date
MY198819A (en) 2023-09-29
EP3383170A1 (en) 2018-10-10
EP3383170A4 (en) 2019-04-17
AU2022218597B2 (en) 2024-12-12
CN116064641A (zh) 2023-05-05
JP2019503666A (ja) 2019-02-14
JP2022023088A (ja) 2022-02-07
RU2018123563A3 (es) 2020-05-15
JP7248337B2 (ja) 2023-03-29
MA43388A (fr) 2018-10-10
US12270038B2 (en) 2025-04-08
KR20180085794A (ko) 2018-07-27
JP2023071957A (ja) 2023-05-23
AU2016362578A1 (en) 2018-07-05
AU2016362578B2 (en) 2022-08-04
WO2017096254A1 (en) 2017-06-08
US11214813B2 (en) 2022-01-04
AU2022218597A1 (en) 2022-09-15
AU2022218597C1 (en) 2025-04-03
CN108463103A (zh) 2018-08-28
CN108463103B (zh) 2022-08-26
US10590429B2 (en) 2020-03-17
JP6956084B2 (ja) 2021-10-27
US20230323377A1 (en) 2023-10-12
RU2018123563A (ru) 2020-01-09
MX2021014746A (es) 2022-01-18
US20250197876A1 (en) 2025-06-19
BR112018011050A2 (pt) 2018-11-21
US20180346917A1 (en) 2018-12-06
US11753649B2 (en) 2023-09-12
HK1258848A1 (zh) 2019-11-22
PH12018501162A1 (en) 2019-01-21
MX388423B (es) 2025-03-19
US20200231980A1 (en) 2020-07-23
US20220340920A1 (en) 2022-10-27
CA3006996A1 (en) 2017-06-08

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