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MX2018005834A - Separacion de proteina mejorada en cromatografia de intercambio de ion. - Google Patents

Separacion de proteina mejorada en cromatografia de intercambio de ion.

Info

Publication number
MX2018005834A
MX2018005834A MX2018005834A MX2018005834A MX2018005834A MX 2018005834 A MX2018005834 A MX 2018005834A MX 2018005834 A MX2018005834 A MX 2018005834A MX 2018005834 A MX2018005834 A MX 2018005834A MX 2018005834 A MX2018005834 A MX 2018005834A
Authority
MX
Mexico
Prior art keywords
ion exchange
exchange chromatography
protein separation
improved protein
preparative
Prior art date
Application number
MX2018005834A
Other languages
English (en)
Inventor
Joehnck Matthias
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of MX2018005834A publication Critical patent/MX2018005834A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/362—Cation-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Peptides Or Proteins (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

La presente invención se refiere a separaciones de proteína preparativas mejoradas (=5 g/l). Estas mejoras se obtienen combinando gradientes de sal y de pH para separaciones de proteína preparativas en combinación con el desarrollo de una separación de proteína de elución por pasos preparativa con base en los datos generados mediante las corridas de gradiente de sal-pH combinadas.
MX2018005834A 2015-11-18 2016-10-28 Separacion de proteina mejorada en cromatografia de intercambio de ion. MX2018005834A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP15195242 2015-11-18
PCT/EP2016/001803 WO2017084737A1 (en) 2015-11-18 2016-10-28 Improved protein separation in ion exchange chromatography

Publications (1)

Publication Number Publication Date
MX2018005834A true MX2018005834A (es) 2018-08-01

Family

ID=54608366

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018005834A MX2018005834A (es) 2015-11-18 2016-10-28 Separacion de proteina mejorada en cromatografia de intercambio de ion.

Country Status (13)

Country Link
US (1) US20180327447A1 (es)
EP (1) EP3377513A1 (es)
JP (1) JP2018538268A (es)
KR (1) KR20180083409A (es)
CN (1) CN108350026A (es)
AU (1) AU2016355739A1 (es)
BR (1) BR112018009881A2 (es)
CA (1) CA3005483A1 (es)
IL (1) IL259180A (es)
MX (1) MX2018005834A (es)
RU (1) RU2018121653A (es)
SG (1) SG11201804082XA (es)
WO (1) WO2017084737A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220017570A1 (en) * 2018-11-05 2022-01-20 Bristol-Meyers Squibb Company Method for purifying pegylated protein
AU2021210905A1 (en) * 2020-01-24 2022-09-01 Mural Oncology, Inc. Methods of purification
JP7686180B2 (ja) * 2020-04-17 2025-06-02 小野薬品工業株式会社 タンパク質製剤原薬の着色除去方法
US20230167153A1 (en) * 2020-05-01 2023-06-01 Kashiv Biosciences, Llc An improved process of purification of protein
US20240383945A1 (en) * 2021-05-10 2024-11-21 Chiome Bioscience Inc. Purification method of antibody composition
WO2024261724A1 (en) * 2023-06-23 2024-12-26 Lupin Limited Ion-exchange chromatography for separation and analysis of charge variants
WO2025160533A1 (en) * 2024-01-26 2025-07-31 Mriglobal Systems and methods for separation of crispr/cas components

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI1308455T1 (sl) * 1998-05-06 2006-08-31 Genentech Inc Sestavek, ki obsega protitelesa anti-HER2
CN1234725C (zh) * 2004-04-07 2006-01-04 陈志南 一种高效快速纯化制备片段抗体的方法
TWI391399B (zh) * 2005-05-25 2013-04-01 Hoffmann La Roche 測定溶離多肽之鹽濃度之方法
ES2596727T3 (es) * 2010-03-30 2017-01-11 Octapharma Ag Procedimiento de purificación de un factor proteico de crecimiento G-CSF
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
US9334319B2 (en) * 2012-04-20 2016-05-10 Abbvie Inc. Low acidic species compositions
BR112015005161A2 (pt) * 2012-09-11 2017-07-04 Coherus Biosciences Inc etanercepte corretamente dobrado em alta pureza e excelente rendimento
JP2015537212A (ja) * 2012-11-15 2015-12-24 ジェネンテック， インコーポレイテッド イオン強度媒介のｐｈ勾配イオン交換クロマトグラフィー
CN104628846B (zh) * 2013-11-06 2019-12-06 三生国健药业（上海）股份有限公司 重组蛋白质的纯化方法
ES2612427T3 (es) * 2014-02-11 2017-05-16 Richter-Helm Bio Tec Gmbh & Co. Kg Método para purificar teriparatida (PTH1-34)
MX2018005831A (es) * 2015-11-18 2018-08-01 Merck Patent Gmbh Gradientes de ph-sal opuestos para separaciones de proteina mejoradas.

Also Published As

Publication number Publication date
CN108350026A (zh) 2018-07-31
AU2016355739A1 (en) 2018-07-05
KR20180083409A (ko) 2018-07-20
WO2017084737A1 (en) 2017-05-26
BR112018009881A2 (pt) 2018-11-13
CA3005483A1 (en) 2017-05-26
RU2018121653A (ru) 2019-12-19
JP2018538268A (ja) 2018-12-27
US20180327447A1 (en) 2018-11-15
RU2018121653A3 (es) 2020-01-31
IL259180A (en) 2018-07-31
SG11201804082XA (en) 2018-06-28
EP3377513A1 (en) 2018-09-26

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