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MX2016011194A - Construcciones de expresion y metodos para seleccionar celulas huesped que expresan polipeptidos. - Google Patents

Construcciones de expresion y metodos para seleccionar celulas huesped que expresan polipeptidos.

Info

Publication number
MX2016011194A
MX2016011194A MX2016011194A MX2016011194A MX2016011194A MX 2016011194 A MX2016011194 A MX 2016011194A MX 2016011194 A MX2016011194 A MX 2016011194A MX 2016011194 A MX2016011194 A MX 2016011194A MX 2016011194 A MX2016011194 A MX 2016011194A
Authority
MX
Mexico
Prior art keywords
expression
host cells
polypeptides
methods
cells expressing
Prior art date
Application number
MX2016011194A
Other languages
English (en)
Inventor
Christel Aebischer-Gumy
Bertschinger Martin
Moretti Pierre
Original Assignee
Glenmark Pharmaceuticals Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glenmark Pharmaceuticals Sa filed Critical Glenmark Pharmaceuticals Sa
Publication of MX2016011194A publication Critical patent/MX2016011194A/es

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/30—Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31—Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/50—Vector systems having a special element relevant for transcription regulating RNA stability, not being an intron, e.g. poly A signal

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La invención se refiere a una construcción de expresión para la expresión soluble de polipéptidos en células huésped y para la expresión de polipéptidos en la superficie de células huésped, utilizando empalme alternativo; la cantidad de expresión de la membrana celular de polipéptidos tal como anticuerpos, fragmentos de anticuerpos y anticuerpos biespecíficos se puede correlacionar directamente con la cantidad de polipéptido soluble expresado; en el caso de anticuerpos biespecíficos, la expresión de la membrana celular de los productos de heterodímero y homodímero se puede correlacionar directamente con la expresión soluble de estos productos, ayudando de este modo a la selección de un clon productor deseado.
MX2016011194A 2014-02-28 2015-03-02 Construcciones de expresion y metodos para seleccionar celulas huesped que expresan polipeptidos. MX2016011194A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14157324 2014-02-28
PCT/EP2015/054331 WO2015128509A1 (en) 2014-02-28 2015-03-02 Expression constructs and methods for selecting host cells expressing polypeptides

Publications (1)

Publication Number Publication Date
MX2016011194A true MX2016011194A (es) 2017-04-06

Family

ID=50184819

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016011194A MX2016011194A (es) 2014-02-28 2015-03-02 Construcciones de expresion y metodos para seleccionar celulas huesped que expresan polipeptidos.

Country Status (15)

Country Link
US (2) US20170350886A1 (es)
EP (1) EP3110950B1 (es)
JP (1) JP7008406B2 (es)
KR (1) KR20160135738A (es)
CN (1) CN106255752A (es)
AU (1) AU2015222065B2 (es)
BR (1) BR112016019832A2 (es)
CA (1) CA2940880C (es)
EA (1) EA035266B1 (es)
IL (1) IL247491A0 (es)
MA (1) MA39502A (es)
MX (1) MX2016011194A (es)
NZ (1) NZ723624A (es)
SG (2) SG11201607114VA (es)
WO (1) WO2015128509A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3320099T5 (da) * 2015-07-09 2024-10-07 Inst Nat Sante Rech Med Membranforankret eller udskilt antistof til ekspression af lentivirale vektorer
CA3026778A1 (en) 2016-06-07 2017-12-14 Max-Delbruck-Centrum Fur Molekulare Medizin In Der Helmholtz-Gemeinschaft Chimeric antigen receptor and car-t cells that bind bcma
US11613573B2 (en) * 2017-04-26 2023-03-28 Eureka Therapeutics, Inc. Chimeric antibody/T-cell receptor constructs and uses thereof
JP2020518622A (ja) * 2017-05-02 2020-06-25 アリゲーター・バイオサイエンス・アーベー Ｏｘ４０およびｃｔｌａ−４に対する二重特異性抗体
US20210363214A1 (en) * 2018-03-01 2021-11-25 University Of Washington Transmembrane polypeptides
KR20210025522A (ko) * 2018-06-25 2021-03-09 유니버시티 오브 워싱톤 강력하고 선택적인 인터루킨 모방체의 드 노보 디자인
MX2021015193A (es) * 2019-06-28 2022-01-18 Hoffmann La Roche Metodo para la produccion de un anticuerpo.
CN117836329A (zh) 2021-07-08 2024-04-05 医学生命科学公司 用于鉴定新型药物和药物靶标的高通量筛选系统
CN113881701B (zh) * 2021-09-08 2024-04-19 山东大学 一种阳性转染细胞亲和分选方法及试剂盒

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8354516B2 (en) * 2006-05-17 2013-01-15 Hoffmann-La Roche Inc. Polypeptide producing cells
US7947495B2 (en) * 2007-11-01 2011-05-24 Abbott Biotherapeutics Corp. Immunoglobulin display vectors
US8969539B2 (en) * 2008-06-11 2015-03-03 Jn Biosciences Llc Expression vectors
WO2014126254A1 (ja) * 2013-02-18 2014-08-21 協和発酵キリン株式会社 膜結合型タンパク質発現ベクター

Also Published As

Publication number Publication date
NZ723624A (en) 2022-07-29
EP3110950A1 (en) 2017-01-04
BR112016019832A2 (pt) 2017-10-17
JP2017508459A (ja) 2017-03-30
EP3110950B1 (en) 2022-09-21
US20170350886A1 (en) 2017-12-07
EA201691548A1 (ru) 2017-02-28
CN106255752A (zh) 2016-12-21
IL247491A0 (en) 2016-11-30
KR20160135738A (ko) 2016-11-28
CA2940880A1 (en) 2015-09-03
CA2940880C (en) 2023-09-26
SG10201913396RA (en) 2020-03-30
MA39502A (fr) 2015-09-03
AU2015222065B2 (en) 2021-05-20
US20190056396A1 (en) 2019-02-21
EA035266B1 (ru) 2020-05-22
JP7008406B2 (ja) 2022-01-25
AU2015222065A1 (en) 2016-09-15
WO2015128509A1 (en) 2015-09-03
SG11201607114VA (en) 2016-09-29

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