Overview
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2705)
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About this book
This volume looks at the latest methods used to study and modulate the biological function and mechanisms of SH2 domains. The chapters in this book are organized into five parts. Part One presents methodology aimed at determining the structures and dynamics of SH2 domains and their complexes with phosphopeptides. Part Two discusses techniques to understand and predict interactions of SH2 domains by measuring or calculating their affinity to phosphopeptides. Part Three focuses on inhibitors of SH2 domains that lead the way for chemical tool development and drug discovery. Part Four describes how to evolve and engineer SH2 domains with specific binding properties, and Part Five explores how to measure the regulation of protein tyrosine phosphatase activity through allosteric binding of peptides to SH2 domains and condensation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, SH2 Domains: Functional Modules and Evolving Tools in Biology is a valuable resource for researchers, working in the biophysical and biochemical field, who want to learn more about this exciting and versatile class of regulatory and signaling domains.
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Table of contents (20 protocols)
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Front Matter
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Structure and Dynamics of SH2 Domains
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Front Matter
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Biophysics and Bioinformatics of SH2 Domain–Phosphopeptide Binding
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Front Matter
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Small-Molecule Binders and Inhibitors of SH2 Domains
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Front Matter
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Engineering SH2 Domains
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Front Matter
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Editors and Affiliations
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Bibliographic Information
Book Title: SH2 Domains
Book Subtitle: Functional Modules and Evolving Tools in Biology
Editors: Teresa Carlomagno, Maja Köhn
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-3393-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2023
Hardcover ISBN: 978-1-0716-3392-2Published: 06 September 2023
Softcover ISBN: 978-1-0716-3395-3Published: 06 September 2024
eBook ISBN: 978-1-0716-3393-9Published: 05 September 2023
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XI, 382
Number of Illustrations: 10 b/w illustrations, 57 illustrations in colour
Topics: Cell Biology, Life Sciences, general, Chemistry/Food Science, general