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SARS-CoV-2

Methods and Protocols

  • Book
  • © 2022

Overview

Editors:
  1. Justin Jang Hann Chu

    1. Department of Microbiology and Immunology; Infectious Disease TRD; BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

  2. Bintou Ahmadou Ahidjo

    1. The University of British Columbia, Vancouver, Canada

  3. Chee Keng Mok

    1. BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2452)

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About this book

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. 

Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and thenovel coronavirus disease that results from it.

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Table of contents (25 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Clinical and Diagnostic Virology

    1. Front Matter

      Pages 1-1
      Download chapter PDF

    2. Evolution and Epidemiology of SARS-CoV-2 Virus

      • Yu-Nong Gong, Kuo-Ming Lee, Shin-Ru Shih
      Pages 3-18

    3. Molecular Epidemiology of SARS-CoV-2 by Sequencing

      • Yan Yan, Qinxue Hu
      Pages 19-32

    4. Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2

      • Xuemei Yang, Ning Dong, Sheng Chen
      Pages 33-43

    5. Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance

      • Vidya Keshav, Lesley Scott, Anura David, Lara Noble, Elizabeth Mayne, Wendy Stevens
      Pages 45-62

    6. Diagnostic Method for COVID-19 Using Sugar Chain –Immobilized Nanoparticles and Saliva Specimens

      • Yasuo Suda, Yasuhisa Tajima, Jun-ichiro Nishi, Takashi Kajiya
      Pages 63-74

    7. Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay

      • Alexander Wilhelm, Christiane Pallas, Rolf Marschalek, Marek Widera
      Pages 75-98

    8. Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans

      • Linda Hueston
      Pages 99-107

  3. Cell Culture, Virus Replication, and Cellular Responses

    1. Front Matter

      Pages 109-109
      Download chapter PDF

    2. Propagation and Quantification of SARS-CoV-2

      • Alexander S. Jureka, Christopher F. Basler
      Pages 111-129

    3. Quantification of Infectious SARS-CoV-2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay

      • C. Korin Bullen, Stephanie L. Davis, Monika M. Looney
      Pages 131-146

    4. One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

      • Raphael Nyaruaba, Xiohong Li, Caroline Mwaliko, Faith Ogolla, Changchang Li, Lu Zhao et al.
      Pages 147-166

    5. Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics

      • Lucia Grenga, Duarte Gouveia, Jean Armengaud
      Pages 167-182

    6. Detection of SARS-CoV-2 by Mass Spectrometry

      • Petra Wandernoth, Katharina Kriegsmann, Jörg Kriegsmann, Mark Kriegsmann
      Pages 183-196

    7. Bioinformatics-Based Approaches to Study Virus–Host Interactions During SARS-CoV-2 Infection

      • Muhammad Saad Khan, Qudsia Yousafi, Shabana Bibi, Muhammad Azhar, Awais Ihsan
      Pages 197-212

    8. Human Nasal Epithelial Cells (hNECs ) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2

      • Kai Sen Tan, Akshamal M. Gamage, Jing Liu
      Pages 213-224

  4. Immunology and Animal Models

    1. Front Matter

      Pages 225-225
      Download chapter PDF

    2. Animal Models of COVID-19: Nonhuman Primates

      • Dhiraj K. Singh, Journey Cole, Ruby A. Escobedo, Kendra J. Alfson, Bindu Singh, Tae-Hyung Lee et al.
      Pages 227-258

    3. Animal Models of COVID-19: Transgenic Mouse Model

      • Jun-Gyu Park, Paula A. Pino, Anwari Akhter, Xavier Alvarez, Jordi B. Torrelles, Luis Martinez-Sobrido
      Pages 259-289
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Editors and Affiliations

  • Department of Microbiology and Immunology; Infectious Disease TRD; BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Justin Jang Hann Chu

  • The University of British Columbia, Vancouver, Canada

    Bintou Ahmadou Ahidjo

  • BSL3 Core Facility, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore

    Chee Keng Mok

Accessibility Information

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Bibliographic Information

  • Book Title: SARS-CoV-2

  • Book Subtitle: Methods and Protocols

  • Editors: Justin Jang Hann Chu, Bintou Ahmadou Ahidjo, Chee Keng Mok

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2111-0

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022

  • Hardcover ISBN: 978-1-0716-2110-3Published: 14 May 2022

  • Softcover ISBN: 978-1-0716-2113-4Published: 14 May 2023

  • eBook ISBN: 978-1-0716-2111-0Published: 13 May 2022

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 478

  • Number of Illustrations: 12 b/w illustrations, 64 illustrations in colour

  • Topics: Virology

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