Overview
- Includes cutting-edge techniques
- Provides step-by-step detail essential for reproducible results
- Contains key implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2170)
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About this book
This updated book covers a wide range of techniques on RNA extraction, detection, quantification, visualization, and genome-wide profiling, from conventional methods to state-of-the-art high throughput approaches. It includes detailed techniques to examine mRNAs, small non-coding RNAs, protein-associated small RNAs, organelle RNAs, endosymbiont RNAs, and alternatively spliced RNA variants from various organisms. RNA editing and the computational data processing for genome-wide datasets is also discussed. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
Authoritative and practical, RNA Abundance Analysis: Methods and Protocols, Second Edition provides a comprehensive set of techniques and methods on isolating and analyzing mRNAs, small RNAs, and other RNA variants, in order to assist researchers in their gene expression studies.
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Table of contents (16 protocols)
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Front Matter
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Back Matter
Editors and Affiliations
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Bibliographic Information
Book Title: RNA Abundance Analysis
Book Subtitle: Methods and Protocols
Editors: Hailing Jin, Isgouhi Kaloshian
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-0743-5
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021
Hardcover ISBN: 978-1-0716-0742-8Published: 15 August 2020
Softcover ISBN: 978-1-0716-0745-9Published: 16 August 2021
eBook ISBN: 978-1-0716-0743-5Published: 14 August 2020
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 2
Number of Pages: XI, 233
Number of Illustrations: 12 b/w illustrations