diff --git a/doc/api/graphql/reference/index.md b/doc/api/graphql/reference/index.md
index d4f9743c6339efc0a2b5e7b84e0b28d27145b756..c1a7d36ae44020fba432466f945eec0a9de678a2 100644
--- a/doc/api/graphql/reference/index.md
+++ b/doc/api/graphql/reference/index.md
@@ -3407,6 +3407,27 @@ Input type: `ProjectSetComplianceFrameworkInput`
 | <a id="mutationprojectsetcomplianceframeworkerrors"></a>`errors` | [`[String!]!`](#string) | Errors encountered during execution of the mutation. |
 | <a id="mutationprojectsetcomplianceframeworkproject"></a>`project` | [`Project`](#project) | Project after mutation. |
 
+### `Mutation.projectSetLocked`
+
+Input type: `ProjectSetLockedInput`
+
+#### Arguments
+
+| Name | Type | Description |
+| ---- | ---- | ----------- |
+| <a id="mutationprojectsetlockedclientmutationid"></a>`clientMutationId` | [`String`](#string) | A unique identifier for the client performing the mutation. |
+| <a id="mutationprojectsetlockedfilepath"></a>`filePath` | [`String!`](#string) | Full path to the file. |
+| <a id="mutationprojectsetlockedlock"></a>`lock` | [`Boolean!`](#boolean) | Whether or not to lock the file path. |
+| <a id="mutationprojectsetlockedprojectpath"></a>`projectPath` | [`ID!`](#id) | Full path of the project to mutate. |
+
+#### Fields
+
+| Name | Type | Description |
+| ---- | ---- | ----------- |
+| <a id="mutationprojectsetlockedclientmutationid"></a>`clientMutationId` | [`String`](#string) | A unique identifier for the client performing the mutation. |
+| <a id="mutationprojectsetlockederrors"></a>`errors` | [`[String!]!`](#string) | Errors encountered during execution of the mutation. |
+| <a id="mutationprojectsetlockedproject"></a>`project` | [`Project`](#project) | Project after mutation. |
+
 ### `Mutation.prometheusIntegrationCreate`
 
 Input type: `PrometheusIntegrationCreateInput`
diff --git a/ee/app/graphql/ee/types/mutation_type.rb b/ee/app/graphql/ee/types/mutation_type.rb
index c5b76077a532ef018c7f04e7e8772de57d3bddf2..7de4261bfdae6c0ff86a760af91102f12089aba6 100644
--- a/ee/app/graphql/ee/types/mutation_type.rb
+++ b/ee/app/graphql/ee/types/mutation_type.rb
@@ -23,6 +23,7 @@ module MutationType
         mount_mutation ::Mutations::Epics::SetSubscription
         mount_mutation ::Mutations::Epics::AddIssue
         mount_mutation ::Mutations::GitlabSubscriptions::Activate
+        mount_mutation ::Mutations::Projects::SetLocked
         mount_mutation ::Mutations::Iterations::Create
         mount_mutation ::Mutations::Iterations::Update
         mount_mutation ::Mutations::Iterations::Delete
diff --git a/ee/app/graphql/mutations/projects/set_locked.rb b/ee/app/graphql/mutations/projects/set_locked.rb
new file mode 100644
index 0000000000000000000000000000000000000000..ee509f3060acb64af27c10e055ddea87a205c0b0
--- /dev/null
+++ b/ee/app/graphql/mutations/projects/set_locked.rb
@@ -0,0 +1,103 @@
+# frozen_string_literal: true
+
+module Mutations
+  module Projects
+    class SetLocked < BaseMutation
+      include FindsProject
+
+      graphql_name 'ProjectSetLocked'
+
+      authorize :push_code
+
+      argument :project_path, GraphQL::Types::ID,
+               required: true,
+               description: 'Full path of the project to mutate.'
+
+      argument :file_path, GraphQL::Types::String,
+               required: true,
+               description: 'Full path to the file.'
+
+      argument :lock,
+               GraphQL::Types::Boolean,
+               required: true,
+               description: 'Whether or not to lock the file path.'
+
+      field :project, Types::ProjectType, null: true,
+            description: 'Project after mutation.'
+
+      attr_reader :project
+
+      def resolve(project_path:, file_path:, lock:)
+        @project = authorized_find!(project_path)
+
+        unless allowed?
+          raise Gitlab::Graphql::Errors::ResourceNotAvailable, 'FileLocks feature disabled'
+        end
+
+        if lock
+          lock_path(file_path)
+        else
+          unlock_path(file_path)
+        end
+
+        {
+          project: project,
+          errors: []
+        }
+      rescue PathLocks::UnlockService::AccessDenied => e
+        {
+          project: nil,
+          errors: [e.message]
+        }
+      end
+
+      private
+
+      delegate :repository, to: :project
+
+      def fetch_path_lock(file_path)
+        project.path_locks.find_by(path: file_path) # rubocop: disable CodeReuse/ActiveRecord
+      end
+
+      def lock_path(file_path)
+        return if fetch_path_lock(file_path)
+
+        path_lock = PathLocks::LockService.new(project, current_user).execute(file_path)
+
+        if path_lock.persisted? && sync_with_lfs?(file_path)
+          Lfs::LockFileService.new(project, current_user, path: file_path, create_path_lock: false).execute
+        end
+      end
+
+      def unlock_path(file_path)
+        path_lock = fetch_path_lock(file_path)
+
+        return unless path_lock
+
+        PathLocks::UnlockService.new(project, current_user).execute(path_lock)
+
+        if sync_with_lfs?(file_path)
+          Lfs::UnlockFileService.new(project, current_user, path: file_path, force: true).execute
+        end
+      end
+
+      def sync_with_lfs?(file_path)
+        project.lfs_enabled? && lfs_file?(file_path)
+      end
+
+      def lfs_file?(file_path)
+        blob = repository.blob_at_branch(repository.root_ref, file_path)
+
+        return false unless blob
+
+        lfs_blob_ids = LfsPointersFinder.new(repository, file_path).execute
+
+        lfs_blob_ids.include?(blob.id)
+      end
+
+      def allowed?
+        project.licensed_feature_available?(:file_locks)
+      end
+    end
+  end
+end
diff --git a/ee/spec/graphql/mutations/projects/set_locked_spec.rb b/ee/spec/graphql/mutations/projects/set_locked_spec.rb
new file mode 100644
index 0000000000000000000000000000000000000000..29ef097956ff68eef19cb29ea8e91fcf9bc94d3e
--- /dev/null
+++ b/ee/spec/graphql/mutations/projects/set_locked_spec.rb
@@ -0,0 +1,174 @@
+# frozen_string_literal: true
+
+require 'spec_helper'
+
+RSpec.describe Mutations::Projects::SetLocked do
+  subject(:mutation) { described_class.new(object: nil, context: { current_user: user }, field: nil) }
+
+  let_it_be(:user) { create(:user) }
+  let_it_be(:project) { create(:project, :repository) }
+
+  describe '#resolve' do
+    subject { mutation.resolve(project_path: project.full_path, file_path: file_path, lock: lock) }
+
+    let(:file_path) { 'README.md' }
+    let(:lock) { true }
+    let(:mutated_path_locks) { subject[:project].path_locks }
+
+    it 'raises an error if the resource is not accessible to the user' do
+      expect { subject }.to raise_error(Gitlab::Graphql::Errors::ResourceNotAvailable)
+    end
+
+    context 'when the user can lock the file' do
+      let(:lock) { true }
+
+      before do
+        project.add_developer(user)
+      end
+
+      context 'when file_locks feature is not available' do
+        before do
+          stub_licensed_features(file_locks: false)
+        end
+
+        it 'raises an error' do
+          expect { subject }.to raise_error(Gitlab::Graphql::Errors::ResourceNotAvailable)
+        end
+      end
+
+      context 'when file is not locked' do
+        it 'sets path locks for the project' do
+          expect { subject }.to change { project.path_locks.count }.by(1)
+          expect(mutated_path_locks.first).to have_attributes(path: file_path, user: user)
+        end
+      end
+
+      context 'when file is already locked' do
+        before do
+          create(:path_lock, project: project, path: file_path)
+        end
+
+        it 'does not change the lock' do
+          expect { subject }.not_to change { project.path_locks.count }
+        end
+      end
+
+      context 'when LFS is enabled' do
+        let(:file_path) { 'files/lfs/lfs_object.iso' }
+
+        before do
+          allow_next_found_instance_of(Project) do |project|
+            allow(project).to receive(:lfs_enabled?) { true }
+          end
+        end
+
+        it 'locks the file in LFS' do
+          expect { subject }.to change { project.lfs_file_locks.count }.by(1)
+        end
+
+        context 'when file is not tracked in LFS' do
+          let(:file_path) { 'README.md' }
+
+          it 'does not lock the file' do
+            expect { subject }.not_to change { project.lfs_file_locks.count }
+          end
+        end
+
+        context 'when locking a directory' do
+          let(:file_path) { 'lfs/' }
+
+          it 'locks the directory' do
+            expect { subject }.to change { project.path_locks.count }.by(1)
+          end
+
+          it 'does not locks the directory through LFS' do
+            expect { subject }.not_to change { project.lfs_file_locks.count }
+          end
+        end
+      end
+    end
+
+    context 'when the user can unlock the file' do
+      let(:lock) { false }
+
+      before do
+        project.add_developer(user)
+      end
+
+      context 'when file is already locked by the same user' do
+        before do
+          create(:path_lock, project: project, path: file_path, user: user)
+        end
+
+        it 'unlocks the file' do
+          expect { subject }.to change { project.path_locks.count }.by(-1)
+          expect(mutated_path_locks).to be_empty
+        end
+      end
+
+      context 'when file is already locked by somebody else' do
+        before do
+          create(:path_lock, project: project, path: file_path)
+        end
+
+        it 'returns an error' do
+          expect(subject[:errors]).to eq(['You have no permissions'])
+        end
+      end
+
+      context 'when file is not locked' do
+        it 'does nothing' do
+          expect { subject }.not_to change { project.path_locks.count }
+          expect(mutated_path_locks).to be_empty
+        end
+      end
+
+      context 'when LFS is enabled' do
+        let(:file_path) { 'files/lfs/lfs_object.iso' }
+
+        before do
+          allow_next_found_instance_of(Project) do |project|
+            allow(project).to receive(:lfs_enabled?) { true }
+          end
+        end
+
+        context 'when file is locked' do
+          before do
+            create(:lfs_file_lock, project: project, path: file_path, user: user)
+            create(:path_lock, project: project, path: file_path, user: user)
+          end
+
+          it 'unlocks the file' do
+            expect { subject }.to change { project.path_locks.count }.by(-1)
+          end
+
+          it 'unlocks the file in LFS' do
+            expect { subject }.to change { project.lfs_file_locks.count }.by(-1)
+          end
+
+          context 'when file is not tracked in LFS' do
+            let(:file_path) { 'README.md' }
+
+            it 'does not unlock the file' do
+              expect { subject }.not_to change { project.lfs_file_locks.count }
+            end
+          end
+
+          context 'when unlocking a directory' do
+            let(:file_path) { 'lfs/' }
+
+            it 'unlocks the directory' do
+              expect { subject }.to change { project.path_locks.count }.by(-1)
+            end
+
+            it 'does not call the LFS unlock service' do
+              expect(Lfs::UnlockFileService).not_to receive(:new)
+
+              subject
+            end
+          end
+        end
+      end
+    end
+  end
+end
diff --git a/ee/spec/requests/api/graphql/mutations/projects/lock_path_spec.rb b/ee/spec/requests/api/graphql/mutations/projects/lock_path_spec.rb
new file mode 100644
index 0000000000000000000000000000000000000000..73826bb83e7ec5d16d76340ed98ead3002ce61fc
--- /dev/null
+++ b/ee/spec/requests/api/graphql/mutations/projects/lock_path_spec.rb
@@ -0,0 +1,68 @@
+# frozen_string_literal: true
+
+require 'spec_helper'
+
+RSpec.describe "Lock/unlock project's file path" do
+  include GraphqlHelpers
+
+  let_it_be(:current_user) { create(:user) }
+  let_it_be(:project) { create(:project) }
+
+  let(:attributes) { { file_path: file_path, lock: lock } }
+  let(:file_path) { 'README.md' }
+  let(:lock) { true }
+  let(:mutation) do
+    params = { project_path: project.full_path }.merge(attributes)
+
+    graphql_mutation(:project_set_locked, params) do
+      <<-QL.strip_heredoc
+        project {
+          id
+          pathLocks {
+            nodes {
+              path
+            }
+          }
+        }
+        errors
+      QL
+    end
+  end
+
+  def mutation_response
+    graphql_mutation_response(:project_set_locked)
+  end
+
+  context 'when the user does not have permission' do
+    it_behaves_like 'a mutation that returns a top-level access error'
+
+    it 'does not create requirement' do
+      expect { post_graphql_mutation(mutation, current_user: current_user) }.not_to change { project.path_locks.count }
+    end
+  end
+
+  context 'when the user has permission' do
+    before do
+      project.add_developer(current_user)
+    end
+
+    it 'creates the path lock' do
+      post_graphql_mutation(mutation, current_user: current_user)
+
+      project_hash = mutation_response['project']
+
+      expect(project_hash.dig('pathLocks', 'nodes', 0, 'path')).to eq(file_path)
+    end
+
+    context 'when there are validation errors' do
+      let(:lock) { false }
+
+      before do
+        create(:path_lock, project: project, path: file_path)
+      end
+
+      it_behaves_like 'a mutation that returns errors in the response',
+                      errors: ['You have no permissions']
+    end
+  end
+end