diff --git a/changelogs/unreleased/119107-respect-dnt-for-experiments.yml b/changelogs/unreleased/119107-respect-dnt-for-experiments.yml
new file mode 100644
index 0000000000000000000000000000000000000000..0132599d40bc817167b6efbc24a23e8f0e74a519
--- /dev/null
+++ b/changelogs/unreleased/119107-respect-dnt-for-experiments.yml
@@ -0,0 +1,5 @@
+---
+title: 'Use DNT: 1 as an experiment opt-out mechanism'
+merge_request: 22100
+author:
+type: other
diff --git a/lib/gitlab/experimentation.rb b/lib/gitlab/experimentation.rb
index 7c59267c0b62111dc9ce7533ea4cc1aea25deb2c..30c8eaf605a88d541a07661c5992537b5eb66d72 100644
--- a/lib/gitlab/experimentation.rb
+++ b/lib/gitlab/experimentation.rb
@@ -40,7 +40,7 @@ module ControllerConcern
       extend ActiveSupport::Concern
 
       included do
-        before_action :set_experimentation_subject_id_cookie
+        before_action :set_experimentation_subject_id_cookie, unless: :dnt_enabled?
         helper_method :experiment_enabled?
       end
 
@@ -56,7 +56,12 @@ def set_experimentation_subject_id_cookie
       end
 
       def experiment_enabled?(experiment_key)
-        Experimentation.enabled_for_user?(experiment_key, experimentation_subject_index) || forced_enabled?(experiment_key)
+        return false if dnt_enabled?
+
+        return true if Experimentation.enabled_for_user?(experiment_key, experimentation_subject_index)
+        return true if forced_enabled?(experiment_key)
+
+        false
       end
 
       def track_experiment_event(experiment_key, action, value = nil)
@@ -73,6 +78,10 @@ def frontend_experimentation_tracking_data(experiment_key, action, value = nil)
 
       private
 
+      def dnt_enabled?
+        Gitlab::Utils.to_boolean(request.headers['DNT'])
+      end
+
       def experimentation_subject_id
         cookies.signed[:experimentation_subject_id]
       end
diff --git a/spec/lib/gitlab/experimentation_spec.rb b/spec/lib/gitlab/experimentation_spec.rb
index 1506794cbb546e16221a3921a3a669a115e84624..a39c50ab038f49fe8d6330f686d99523f127fb7b 100644
--- a/spec/lib/gitlab/experimentation_spec.rb
+++ b/spec/lib/gitlab/experimentation_spec.rb
@@ -30,7 +30,12 @@ def index
     end
 
     describe '#set_experimentation_subject_id_cookie' do
+      let(:do_not_track) { nil }
+      let(:cookie) { cookies.permanent.signed[:experimentation_subject_id] }
+
       before do
+        request.headers['DNT'] = do_not_track if do_not_track.present?
+
         get :index
       end
 
@@ -46,12 +51,30 @@ def index
 
       context 'cookie is not present' do
         it 'sets a permanent signed cookie' do
-          expect(cookies.permanent.signed[:experimentation_subject_id]).to be_present
+          expect(cookie).to be_present
+        end
+
+        context 'DNT: 0' do
+          let(:do_not_Track) { '0' }
+
+          it 'sets a permanent signed cookie' do
+            expect(cookie).to be_present
+          end
+        end
+
+        context 'DNT: 1' do
+          let(:do_not_track) { '1' }
+
+          it 'does nothing' do
+            expect(cookie).not_to be_present
+          end
         end
       end
     end
 
     describe '#experiment_enabled?' do
+      subject { controller.experiment_enabled?(:test_experiment) }
+
       context 'cookie is not present' do
         it 'calls Gitlab::Experimentation.enabled_for_user? with the name of the experiment and an experimentation_subject_index of nil' do
           expect(Gitlab::Experimentation).to receive(:enabled_for_user?).with(:test_experiment, nil)
@@ -72,11 +95,25 @@ def index
         end
       end
 
+      it 'returns true when DNT: 0 is set in the request' do
+        allow(Gitlab::Experimentation).to receive(:enabled_for_user?) { true }
+        controller.request.headers['DNT'] = '0'
+
+        is_expected.to be_truthy
+      end
+
+      it 'returns false when DNT: 1 is set in the request' do
+        allow(Gitlab::Experimentation).to receive(:enabled_for_user?) { true }
+        controller.request.headers['DNT'] = '1'
+
+        is_expected.to be_falsy
+      end
+
       describe 'URL parameter to force enable experiment' do
-        it 'returns true' do
+        it 'returns true unconditionally' do
           get :index, params: { force_experiment: :test_experiment }
 
-          expect(controller.experiment_enabled?(:test_experiment)).to be_truthy
+          is_expected.to be_truthy
         end
       end
     end