Ferroptosis is a non-apoptotic type of cell death that is induced by uncontrolled iron-dependent phospholipid peroxidation and has important roles in disease. This Expert Recommendation article summarizes ferroptosis regulation and mechanisms and provides recommendations to increase the reproducibility and robustness of ferroptosis research.

Membrane contact sites between organelles facilitate the exchange of ions, lipids and metabolites. This Expert Recommendation highlights recent advances in defining the organelle contactome and discusses challenges and future directions in membrane contact site research.

Nuclear speckles support the splicing of levelled exon–intron architecture transcripts, and their evolution shaped genome organization in amniotes.

In this Tools of the Trade article, Ma (Dai lab) discusses the design of synthetic intrinsically disordered proteins that can be used to regulate the solubility of intracellular proteins, thereby affecting their activity.

This Comment discusses the bidirectional interplay between STING signalling and lipid homeostasis, highlighting the emerging therapeutic relevance of this regulatory network.

Yang and Liu discuss strategies that help address particle orientation bias in cryogenic electron microscopy (cryo-EM), and offer practical advice to tackle it.

Cytoplasmic partitioning in early embryos depends on microtubule asters. A study reveals that different species use distinct mechanisms to stabilize these inherently unstable structures and preserve robust cytoplasmic compartments.

Based on microRNAs, DIMMERs is a modular tool for dampening expression fluctuations of multiple proteins in a cell owing to transgene copy number variations.