According to Brian Yuzna's commentary, Trimark did not require the film to use the same actors or have the same comedic elements as the previous two films but they did require the zombies (including Julie) crave and eat brains.
An entire day of footage, according to actor J Trevor Edmund, was lost because camera assistants never "checked the gate," i.e. never checked to see if the lenses was clear of hair or debris.
Make up artist Tom Rainone claims Clarence Epperson, the first animated cadaver in the film, was a homeless vagrant who demanded more money and limousine escorts to the set as the shoot got closer.
Brian Yuzna was disappointed with the lack of screen time for his previous female monster creation in Bride of Re-Animator and wanted Julie to have a far bigger presence.
Shortly after the film's release, Brian Yuzna had expressed interest in writing a fourth ROTLD sequel. It was to have taken place directly after the events in 3, with zombies having somehow escaped the military base and running amok in Los Angeles.