Casting for the film involved Gino Turini who put in part of the money for the film and Hélène Rémy as the film was originally going to be a co-production deal with France. Writer Ernesto Gastaldi once noted that the casting of Tina Gloriani was due to her being the director's lover at the time.
The film was shot at the castle of Artena, in later 1959 in three weeks. Renato Polselli has claimed that the skeletons that in the vampire's crypt scenes were real skeletons. The shot of the vampire's face deteriorating was a homemade special effect. Polselli stated that the case was made with plaster, followed by the make-up artist molding an adhesive rubber mask over it with a layer of ash between the plastic and rubber. "We made a face cast with plaster, then the make-up artist and I molded an adhesive rubber mask over it. Our trick was to put a layer of ash between the plaster and the rubber."
Italian censors gave The Vampire and the Ballerina a V.M. 16 rating, making it "forbidden to those under 16 years old". The censors demanded that all close-ups of the vampire's face be cut and that the final melting of the vampires be shortened. Months after the censors demands were sent, the producer submitted a new version of the film which passed with a V.M. 16 rating and no cuts.
The film has been released for home viewing by Amazon Instant Video and on an English-friendly DVD by NoShame in Italy. Scream Factory released the film on Blu-ray for the first time in the U.S. on May 22, 2018 with a new high definition transfer of the film sourced from the last surviving film elements.
The film's story and screenplay were written by director Renato Polselli and screenplay authors Giuseppe Pellegrini and Ernesto Gastaldi. The original screenplay for the film was written by Giampaolo Callegaris. Ernesto Gastaldi described the script as "rather canine", and wrote a new one with director Renato Polselli. Gastaldi felt the script was no different than any others he had worked on, with the only new element being vampires. Gastaldi commented that since Dracula starring Christopher Lee had been such a big hit in Italy, producers and distributors were eager to make their own vampire films.