The first 3D video game to use the cast from As Novas Aventuras do Batman (1997) for original in-game and cutscene dialogue.
This game is based on As Novas Aventuras do Batman (1997) and takes place after flashback seen in Old Wounds (1998), but before Sins of the Father (1997). Plot points that support this include Mr. Freeze still posing as a threat (as his appearance in Cold Comfort (1997) was the last time he was seen prior to Meltdown (1999)), as well as the fact that Batgirl is the Dark Knight's only active sidekick in the game.
The Joker is still seen in his New Batman Adventures redesign, despite having been re-redesigned for Batman Beyond: Return of the Joker.
Gotham's Mayor Hamilton Hill's appearance in this game marks the first time the character appeared in a video game. Lloyd Bochner voices Mayor Hill in the game, reprising his role from As Novas Aventuras do Batman (1997).