Over 30 members of the cast and crew were temporarily blinded by the misuse of UV lights in the vampire autopsy scene.
Donnie Yen, a martial arts star who plays a member of the Bloodpack (Snowman), was also the film's fight choreographer.
The scene where Reinhardt, who based his character on a Neo-Nazi, asks if Blade if he can blush is based on a real incident that Wesley Snipes experienced. Snipes requested this be inserted into the movie to add to the antagonism between the characters.
(at around 25 mins) When Overlord Damaskinos mentions the virus that causes vampirism, he states it is a type of "parvovirus", though the closed captioning says "horrible virus" because of the obscurity of the term. Parvovirus is an actual family of viruses, and the B13 strain afflicts humans. It meets all the requirements for creating vampires in a fictional environment, since the B13 strain actually does cause sensitivity to daylight in some, it infects the blood & bone marrow, and can be transmitted by saliva. Since most people outside of medicine are unfamiliar with the term, however, they may have also heard "horrible virus".
Wesley Snipes: [Sun Tzu] (at around 1h 8 mins) Snipes' quoting of Sun Tzu - "Keep your friends close, and your enemies closer," follows references to the works of Sun Tzu in many other Snipes movies including Passageiro 57 (1992), Sol Nascente (1993) and A Arte da Guerra (2000).