In order to prepare for their roles, several cast members watched a number of real autopsies.
Most of the organs seen in the movie came from pigs and cows.
The city was identified as Philadelphia in an earlier draft of the script.
Milo Ventimiglia has stated he spent a lot of time at the coroner's office in preparation for the role, including having watched "nineteen autopsies, saw a couple hundred dead bodies -- I mean, anything from a five month-old baby to an 88-year-old man who was stabbed with a screwdriver."