This is the fifth series from DreamWorks Animation Television to not be based on an established property. The other 4 are The Mighty Ones, Gabby's Dollhouse, Dew Drop Diaries, and Curses.
According to an Instagram post from Curses! staff writer Dimitry Pompée, the show is set in the same universe as DreamWorks' 2000 film, The Road to El Dorado, centuries after Miguel and Tulio's adventure. A map to El Dorado is shown in "The Ottoman Ship Helm" featuring a large fish that is also present on the map from the movie.
This is the fifth DreamWorks show after The Mighty Ones, Gabby's Dollhouse, Dew Drop Diaries, and Fright Krewe to not be based on an established property.