Superman (Danny Dark) and Robin (Casey Kasem) were the only two characters voiced by the same performer in every Super Friends series.
The characters of Plastic Man and Green Arrow made their first animated appearances on this show.
Adam West who performed as Batman in Batman (1966) lent his voice to animated depictions of the title character in Super Friends (1973) and Super Friends: Galactic Guardians (1985). He performed that same role in Legends of the Superheroes (1979).
The characters of Wendy, Marvin, and Wonder Dog were introduced to the DC Comics universe over 30 years later in Geoff Johns's Teen Titans series around 2006. Marvin would be subsequently killed off by a rabid Wonder Dog, no less and Wendy made paraplegic by the writer who took over after Johns left the title two years later.
In an effort to add racial diversity, Black Vulcan, Samurai, and Apache Chief were created specifically for the show. Black Vulcan is black, Samurai is Japanese, and Apache Chief is an American Indian. 25 years later, the Justice League comic writer Joe Kelly, a fan of the show, brought the character of Apache Chief to the comic book world. Hanna-Barbera Productions had originally planned to use Black Lightning, DC Comics first prominent black superhero, for the African-American character. But the company learned that, in order to use the character, it would have to pay a royalty fee to Tony Isabella, the writer who had created Black Lightning and wrote the "Black Lightning" comic book. Instead, the artists created Black Vulcan, whose powers and costume were similar to Black Lightning's, in order to avoid paying the fee. In response, Tony Isabella wrote a story in the comic book that featured a con artist named Hanna-Barbera and an impostor pretending to be Black Lightning.