With this game, Mario and Luigi were recast, making it the first mainline Super Mario game to not have Charles Martinet as their voice actor.
This game marks the first time Daisy is playable in a mainline Mario game as she was only playable in Mario spin-off games like Mario Kart, Mario Party and Mario sports games.
The first Mario game where Kevin Afghani is voicing Mario and Luigi.
The Flower Kingdom, the game's main setting, has the same name as a kingdom mentioned in the Super Mario Bros. OVA (1986). This worried fans that Prince Haru, a character from the OVA, would steal Peach from Mario again, while being disguised as Prince Florian. This theory was eventually debunked, as Florian was revealed to be a completely different character, who is naturally a caterpillar.
First Mario game where Princess Daisy isn't voiced by Deanna Mustard.