Trivia

(at around 59 mins) The line that Inmate 333 says while they are all watching The Human Centipede films, "this trash belongs to a world where stars don't shine" was originally written by Roger Ebert in his review for El ciempiés humano (2009).

The film marks one of the final performances by Dieter Laser before his death in 2020.

Dieter Laser and Laurence R. Harvey, who played the villains from the first and second movies, appear here as different characters.

Bill Hutchens, the actor who played Dr. Sebring in part two, also played Inmate 488 in part three.

Akihiro Kitamura played Katsuro in part I and Inmate 333 in part III.