This game is based on Season 3 of Las Tortugas Ninja (2003) the animated series with stages in the game based on following episodes: Space Invaders: Part 1 (2004), Space Invaders: Part 2 (2004), Space Invaders: Part 3 (2004), Bishop's Gambit (2005), Worlds Collide: Part 1 (2004), Worlds Collide: Part 2 (2004), Worlds Collide: Part 3 (2004), New Blood (2005), Exodus: Part 1 (2005), Exodus: Part 2 (2005), Reality Check (2005), Across the Universe (2005), Same as It Never Was (2005), Touch and Go (2004), The Real World: Part 1 (2005) and The Real World: Part 2 (2005).
This is the final Teenage Mutant Ninja Turtle game to be based directly off the Las Tortugas Ninja (2003) animated TV series. This is also the final Teenage Mutant Ninja Turtles video game to produced and distributed by Konami who had had produced and distributed every video game based on the characters at the time.
This is the first Teenage Mutant Ninja Turtles video game to automatically feature all 4 Turtles during game play. Formerly all 4 Turtles could appear in the game, but a player had to control them. This is the first one where CPU controlled Turtles appear and fight alongside a player-controlled Turtle if there are less than 4 players. Also, players can switch control among the 4 Turtles between stages.