The story takes place in Spain, and the Spanish spoken by the villagers in the game is called Castilian (which has a different pronunciation than the standard form of Spanish, known as Español, commonly spoken by many Latino). The currency used throughout the game is the Peseta, which some may find curious, as Spain had switched to Euros in 2002, and the game takes place in 2004. But this may have been done to show how behind the times the area is compared to the rest of Spain, due to its remoteness and its inhabitants being afflicted with the "Plaga" parasite, transforming them into, essentially, mind-controlled puppets. As such, it would make perfect sense for them to still have the older currency in their pockets, etc., because they've all been in their "puppet-like" state for at least two years.
This is the first Resident Evil game where Umbrella isn't responsible for the monsters and bio organic weapons (BOW) creations. Umbrella is gone by this point, and may yet come back.
This Resident Evil used all new enemies that were not in any of the previous games. No zombies, no Cerberus, no crows, no giant spiders, no tyrants, no hunters, no lickers made it to this installment.
The Matilda handgun was named after the character with the same name from Luc Besson's movie El perfecto asesino (1994), which also had a main character called Leon. The game also contains an unlockable "Professional" difficulty in homage to that movie. Furthermore, the menu screen of the 'Separate Ways' missions (added to later versions of game) looks very similar to the poster of Besson's film Nikita (1990), with both protagonists sitting and holding a gun in similar positions.
Ashley will cover her skirt and yell, 'Oh, you pervert!' if the player aims upwards towards her while on lower ground.