Though he was briefly referenced in two episodes, Bugs Bunny did not make an appearance on this show.
This show's theme song was sung by Sir Tom Jones, in a style reminiscent to his performance of the theme song from the James Bond movie Operación trueno (1965). Jones also appeared in caricature form in season two, episode four Talent Show a Go-Go/The Love of a Father (2004) to sing his signature song, "It's Not Unusual".
Many other familiar characters from the Looney Tunes pantheon were featured on this show, often given traits to fit within Duck Dodgers' own universe. For example: Yosemite Sam became "K'chutha Sa'am", a parody of Klingons on Viaje a las estrellas (1966). Elmer Fudd became a parasitic mind-altering alien disease known as The Fudd (2004) though more closely resembling The Borg in Viaje a las estrellas: La nueva generación (1987). Taz was a barbaric ship Captain forced into single combat with Dodgers (a parody of the Star Trek: The Original Series (1966) episode "Arena". Wile E. Coyote showed up as a Predator-like alien hunting the Martian Commander.
This is the first Looney Tunes franchise to be aired on Cartoon Network before Kids' WB went defunct in 2008.
Of the five main characters on this show, four of them appeared in Pato Dodgers en el Siglo 24½ (1953): Daffy Duck (Duck Dodgers), Porky Pig (The Eager Young Space Cadet), Marvin the Martian (Martian Commander X-2), and Dr. I.Q. Hi (Himself). However, the fifth major character, The Martian Queen, was created specifically for the series.