In one of the levels, a female hostage is found trapped in a alien type gel. When you get close to her, she lets out a faint, "Kill me." This idea was sequenced directly from Alien 2: El regreso (1986). By killing the girl, an alien appears, just like in the film.
Is the first game to feature full working mirrors.
Duke's famous quotes about "kicking ass and chewing bubblegum" and "hail to the king" come from horror movies Sobreviven (1988) and Guerrero de las sombras (1992), respectively.
The "Chaingun Cannon" in reality is a Russian made Pribor-3B Nordenfelt gun.
The game cover is a parody of "Army of Darkness", while Duke Nukem poses as Ash.