A portrait of the Mona Lisa can be seen in Spider's house.
This movie uses both real life environments and animation.
The black ant hill is shaped like Minas Tirith and would later be the site of the battle between the black and red ants.
The opening's car is a New Beetle, successor of the Beetle, whose french name means "Ladybug", like the main character.
1st time in the franchise human faces are clearly seen.