The film contains many references to Anaconda 2: the plot is developed around the bloody orchid, one of the main characters is fatally bitten by a spider, the plot indirectly involves the company Wexel hall, the protagonists survive by blowing up the Anaconda and the design of the Anaconda is openly inspired by the green Anaconda.
During the filming of some scenes in the forests, the production got into legal problems because they were filming in a wild protection area and the noise that was generated bothered the local fauna.
The first Anaconda film to have returning characters.
David Hasselhoff was originally going to return.
The Blood Orchid from Anacondas - Die Jagd nach der Blut-Orchidee (2004), is the flower mentioned in this film.